Isolation and Characterization of Antioxidants from Six Indian Medicinal Plants With Reference to Its Wound Healing and Antigenotoxic Activities In Animal Models

Abstract

newline The preliminary phytochemical analysis of the crude extracts of the six newlineselected Indian medicinal plants indicated the presence of major phytochemical newlinecompounds, including phenolics, alkaloids, glycosides, flavonoids, and tannins newlinewhich may be responsible for the observed antioxidant activities, antimicrobial newlineactivities, wound healing activities and antigenotoxic activities. The observed newlineresults further support the view that some traditionally used Indian medicinal newlineplants are promising sources of potential antioxidants and medicinal compounds. newlineA fair correlation between total phenolic content and antioxidant activity newlinewas observed in the six medicinal plant extracts. These observations clearly newlineindicate a close relationship and linkage between phenolics and antioxidant newlineactivity. Plant phenolics constitute one of the major groups of compounds that act newlineas primary antioxidants or free radical scavengers. newlineThe methanol extract of the six Indian medicinal plants was observed to newlinecontain antimicrobial properties. The antimicrobial activities of the six Indian newlinemedicinal plants may be attributed to the presence of major phytochemical newlinecompounds, including phenolics, alkaloids, glycosides, flavonoids, and tannins. newlineIn order to evaluate the wound healing activity of the six Indian medicinal newlineplants, five different extracts were prepared from the leaves of the plants. newlineMethanol, ethanol, ethyl acetate, hexane and chloroform were used for the newlineextraction of the active ingredients. Excision model of wound was made on Swiss newlinealbino mice to assess the wound healing activity of the leaves. Remarkable wound newlinehealing activity was observed with the ointment formulation of the methanol newlineextract at 1% concentration. Wound contraction was calculated as percentage of newlinethe reduction in wound area. A specimen sample of tissue was isolated from the newlinehealed skin of each group of mice for the Histopathological examination.