Preparation characterization and bio efficacy evaluation of peptide fractions from high altitude medicinal mushrooms Cordyceps Sinensis and Ganoderma Lucidum

Abstract

Medicinal mushrooms thriving at cold, arid higher altitudes consist of several metabolites that newlineproffer them with sustainability to adapt to adverse climatic conditions. Therapeutic compounds purified from such medicinal mushrooms have been reported to be highly beneficial for human health improvement. In the present research work, preparation, characterization and bio efficacy evaluation of peptide fractions from mushrooms Cordyceps sinensis and Ganoderma lucidum has been described. Gel filtration chromatography yielded distinct peptide fractions that were characterized by various analytical techniques such as MALDI-MS, HPLC, HPTLC and circular dichroism. The sequences of the most potent bioactive fractions of both medicinal mushrooms were determined by N-terminal sequencing. These fractions possessed substantial antioxidant potential manifested by free radical scavenging and metal chelating activities. Also, effective antibacterial action was observed against Salmonella typhi and Escherichia coli. Peptide fractions from aforesaid mushrooms showed promising protective action against simulated hypobaric hypoxia conditions, both in vitro in human embryonic kidney (HEK 293) cells as well as in vivo in male Sprague Dawley rats. newlineChanges brought about in characteristic hypoxia response markers namely, HIF-1and#945;, VEGF, NFand#954;-B, etc., upon supplementation with specific doses of the peptide fractions were evaluated in HEK 293 cells. Also, modulation mediated by C. sinensis and G. lucidum peptide fractions in varied hematological parameters along with oxidative stress markers like malondialdehyde and reduced glutathione in different tissues of the experimental rats were meticulously observed. Conclusively, the sanguine bioefficacy exhibited by these medicinal mushrooms could be further explored as effective pharmaceutical leads. newline newline