Assessment of functional diversity of Pseudomonas species isolated from rhizosphere of cotton plants

Abstract

Rhizosphere is the site where plant roots, microorganisms and soil components were newlineinteracted together. The plant root exudates and abiotic factors were responsible for diversity newlineof microorganisms. The microorganisms which resides in rhizosphere and promote plant newlinegrowth are called as Plant Growth Promoting Rhizobacteria (PGPR). These PGPR promotes newlinethe plant growth by 3 different mechanisms as, by the production of phytohormones or newlinerecycling the nutrients, by the production of plant pathogen growth inhibiting compounds or newlineby providing signalling to the plants i.e. Induced Systematic Resistance (ISR). Many newlinebacterial genera were studied and identified as Plant Growth Promoting Rhizobacteria but newlineamong them Pseudomonas and Bacillus were dominating one. Cotton is one of the newlineeconomically important plant which was cultivated in 70 countries of the world with a total newlinecoverage of 32.30 m ha. Area wise, India ranks first in global scenario (about 33% of the newlineworld cotton area). However, in production it ranks second next to China. The various biotic newlineand abiotic factors can influence over yield of cotton plant. Majorly insects, fungi, newlinenematodes and some bacteria were responsible for the yield loss (80%) of the cotton plant. newlineThe introduction of BT and HTBT technology initially was found as promoising tool for newlineincrease in crop yield but within a decade the adverse effects were observed which finally newlineresultant into loss of yield. newlineThe gene manipulation technique was not found as effective hence we had to retrieve the newlineold technique with some new tools. This study was just an attempt to use the most studied newlinePGPR Pseudomonas species with AgNPs as new tool. The Pseudomonas species were newlineisolated from monocropic rhizosphere of cotton plant and studied for its functional newlineexpression profiling, antifungal activity and plant growth promotion activity. The newlinecombinatory effect of AgNPs was also evaluated for antifungal, plant growth promotion and newlinecommunity stability activity. The AgNPs exert positive effect over antifiungal and plant newlinegrowth p