In Silico Characterization of 5 HT 2A Receptor Involved in Schizophrenia and its Potential as Therapeutic Target

Abstract

newline5-HT2A receptor is primarily involved in uptake and transport of serotonin molecule from nerve cell to extracellular fluid. In the present study we characterise 5-HT2A receptor by using different in-silico methods. The study involved 5-HT2A homology Model prediction by: Modeller 9.14, Swiss Model server, Phyre2 and Geno3D. The homology models of 5-HT2A were evaluated by Procheck, ERRAT, Qmean score and Ramachandran Plot analysis.During this study we also compared all different models. Further structural analysis suggested that the structure of 5HT2A is a monomer with 18 alpha helices, 7 beta sheets and 1 disulphide bridge. There was no signal peptide region in the protein sequence.The structure contains mostly polar and aromatic amino acid as reported by hydropathy plot. However, in both the partioning system bilayer to water and water to bilayer, there were some hydropathy predicted segments, which were also transmembrane segments. Finally the pore features including diameter profile, size and shape were determined by porewalker. The shape of the pore was found to be conical frustum, conical and cylinder (UDSD).The study clearly suggested that 5-HT2A receptor interaction with its natural ligand serotonin and other inhibitor compounds which provide more information about GPCRs. The 5-HT2A receptor could be an important target for specific treatment for future drug discovery and therapeutic development. newlineKeywords:- 5-HT2A receptor, Homology Modelling, Ramachandran Plot, Profunc, Hydropathy Plot newline