Antihyperlipidemic effects of polyterbal preparation in high fat diet induced hyperlipidemic rats

Abstract

Aqueous plant extracts of Commiphora mukul (gum resin), Cedrus deodara (wood), Bauhinia variegate (bark), Achyranthus aspera (plant), Picrorhiza kurroa (root) were selected for the development of polyherbal preparation. The present study is to evaluate the Anti-hyperlipidemic properties of combined plant extracts comparing it with control and a standard group of studies by the oral route of administration in high-fat diet-induced hyperlipidemic rats and to find out the suitable dose for the hyperlipidemic activity. Individual plant extracts were subjected for the identification of bioactive phytocompounds using HPTLC and winCATS software. Results confirmed the presence of particular biomarkers in individual plant extracts while performing standardization. i.e. Guggulsterone E and Guggulsterone Z in Commiphora mukul (gum resin); Quercetin in Cedrus deodara (wood); and#946;-Sitosterol in Bauhinia variegate (bark); Betaine in Achyranthus aspera (plant); Picroside - I and Picroside - II in Picrorhiza kurroa (root). Development of polyherbal preparation has been done by combining all aqueous plant extracts as per ratio. An acute oral toxicity study as per OECD Guideline 425 has been performed for polyherbal preparation. A detailed behavioral study was done for 14 days and on the 7th and 14th day hematological parameters, biochemical analysis has been performed. Organ weight analysis and histopathology of organs were carried out after 2 weeks. The results of test animals were compared to that of the control animals. No concrete evidence of toxicities attributable to treatment with the polyherbal preparation was observed. Therefore, single oral administration of polyherbal preparation up to 5,000 mg/kg b.wt. to rats under this study condition produced no significantly toxicological effects. Thus, two dose levels were selected in such a way that, the high dose was approximately 1/10th of the maximum dose of 5000 mg/kg (500 mg/kg) and the low dose which was 50% of 1/10th (250 mg/kg) for hyperlipidemic activity in animals.