Anticarcinoma Potential of Asparagus Racemosus extract on Ovarian Carcinoma Skov3 Cell Line

Abstract

Ovarian carcinoma can be classified into at least five dissimilar newlinehistological subtypes that have diverse identifiable risk factors, clinical newlinefeatures, molecular compositions, cells of origin, and treatments. Ovarian newlinecarcinoma is a universal problem, is usually diagnosed at the late stage and newlinehas no effective screening strategy. Standard treatments for carcinoma newlinediagnosis consist of platinum-based chemotherapy and cytoreductive newlinesurgery. In recurrent carcinoma, anti-angiogenic agents, chemotherapy, and newlinepoly [ADP-ribose] polymerase inhibitors are used, and immunological newlinetherapies are presently being tested. Overcoming drug struggle in ovarian newlinecarcinoma is the overarching goal in gynecologic oncology. Hence growth newlineof new effective and affordable anticarcinoma drugs is the constant newlinedemand for these days. Natural plants pave the new pathways in the newlineprogression of new anti-ovarian carcinoma drugs by its various secondary newlinemetabolites. A. racemosus is an important traditional plant which possess newlinevarious pharmacological activities such as lactogenic, antiinflammatory, newlineantioxidant, antidiabetic, antiarthritic and antitumour properties. Due to newlinelack of literatures for A. racemosus plant against ovarian carcinoma newline newline vi newlinetreatment, present study is an effort that has done to analyse the newlineanticarcinoma potential of A. racemosus plant extract against SKOV3, newlinehuman ovarian carcinoma cell line. Plant extract was prepared by cold newlinemaceration method. Phytochemical and HPTLC analysis reveals presence newlineof various secondary metabolites. MTT assay reveals the cytotoxic newlinepotential of ARE extract. ARE extract showed increased cell apoptosis newlinewhich was evidenced from AO/EB and annexin V staining. Cell newlinefragmentation in SKOV3 was increased upon ARE treatment. ARE newlinetreatment induce several cell morphological and nuclear changes in ovarian newlinecarcinoma cells and these findings were comparable with anticarcinoma newlinedrug cisplatin. Therefore, aqueous extract of A. racemosus can be newlineconsidered as a potential candidate for ovarian carcinoma treatment. newline newline