Effect of Ganoderma Extracts Against Clinically Important Biofilm Producing Fungal Isolates from Immunocompromised Patients

Abstract

ABSTRACT Ganoderma lucidum has been used for several decades for both treatment strategy and health promotion. The manifestation of drug resistant, new upcoming infections, elevated costs of readily available drugs has lead to amplify in the employ of conventional medicine worldwide. The present study was aimed to screen the antifungal and antibioflim activity of the Ganoderma lucidum for the DMSO and ethanolic extracts, which could be supportive for the expansion of new tools as antifungal agents for the control of infectious diseases. The present study is a Cross-sectional study, during the July 2013 to September 2017. A total of 350 clinical samples were collected among that 270 samples were shown positivity of fungal growth. All the samples were screened for routine investigations like, microscopic, macroscopic, biochemical tests, enzyme assay to identify the clinically fungal and it species. RPMI 1640- XTT assay was used in this study to determine the fungal bioflim and to check the antifungal and antibioflim activity of different concentrations of both commercially avaiable drugs and the extracts of the Ganoderma. The outcome of the present study clearly explores the importance of the bioflim and its drug resistance. Four major traizole drugs were used to compare with the Ganoderma extract. Commerically avaible drugs like Fluconazole, Itraconazole, Voriconazole and Posaconazole were used to screen the antifungal against the various isolates like Candida albicans, Aspergiullus fumigatus, Aspergillus niger, Aspergillus flavus and Cryptococcus neoformans. Based on the disk diffusion method, the result of antifungal activity was fluconazole were shown resistance, newlinevii newlineItraconazole and Voriconazole were shown least sensitivity and Posaconazole wer shown good significant acitivity agaisnt the all isolates in this study. XTT assay helps to promote the growth of Bioflim production and screened the antibioflim suscepitibility testing. Among result both the extract were shown (Plt0.05) significance, especillay DMSO extract. Were commercially avaible drugs were shown least significance were compared to the Ganoderma Extracts. The present study also deals with the phytochemical analysis and its impiortance in the drug resistance. Biofilms are constantly headstrong to drug treatment for a multiplicity of reasons, and thus can basis for recurrent infections. Triazole drugs like Fluconazole, Itraconazole, Voriconazole and Posaconazole, the most of the common and recent drug used to treat the fungal infections. Overall, posaconazole was higher in falling IFIs and all-cause deaths among patients getting chemotherapy treatment. Ganoderma lucidum is a macro edible mushroom which is exclusive in that its pharmaceutical effect along with nutritional value is higher. Posaconazole were active against to inhibit both fungal growth and bioflim producers. Whereas according to the Ganoderma extracts DMSO were explores the majority of the inhibition of both fungal growth and bioflim producers. newline