Study on muscle Transcriptome of anadromous Indian shad Tenualosa ilisha

Abstract

Tenualosa ilisha (Hamilton, 1822) Popularly known as hilsa, belongs to subfamily newlineAlosinae, family Clupeidae, order Clupeiformes, is one of the most important tropical fishes of newlinethe Indo-Pacific region and has occupied a top position among the edible fishes owing to its newlinetaste, flavor and culinary properties. newlineResearch problem newlineGrowth in fishes is primarily dependent on an increase in skeletal muscle mass. newlineSkeletal muscle is the largest organ system in fish and represents the edible part. The skeletal newlinemuscle of Hilsa is divided into white (65-70%) and dark muscle (30-35%) of total muscle newlinemass and constitutes the 34-48% of the total body weight. It is rich in lipids, particularly mono newlineand poly unsaturated fatty acids, presence of which is supposed to be basis of the unique taste newlineof Hilsa. Muscle proteins are the important storehouse of many of the biological information. newlineHence to study the transcriptome analysis of muscle through next generation sequencing is newlineimportant as it provide genome-wide transcriptome profile and also give useful information to newlinestudy the genes involved myogenesis, lipid synthesis and deposition, immune protection newlineagainst infections. Most fishes continue to grow throughout their but it is also evident that the newlinegrowth rate of Hilsa tends to be slow and steady as the fish gets older. It is necessary to study newlinethe variations in the sequence of some genes related to growth to distinguish fast and slow newlinegrowing fishes. The function of a protein is based on its structure, which is determined by newlinearrangement of amino acid sequences. The amino acid sequence provides most of the newlineinformation required for determining and characterizing the molecule s function, physical and newlinechemical properties. The in silico investigation is needed for characterization of transcript and newlineits deduced protein of Hilsa to understand its mechanism in growth and association of its newlinesequence variation with pattern in growth rate. Taken together the study of transcriptome and newlinein silico characterization is useful for understanding grow