Evaluation Of Antiasthmatic Activity Of Some Plants Of Chhattisgarh Region

Abstract

Asthma is a multifactorial chronic inflammatory disorder of airways, affecting more than newline300 million individuals. The disease is considered an inflammatory disease in the airway, newlineleading to airway hyperresponsiveness, obstruction, mucus hyper-production, and airway newlinewall remodeling. There is compelling evidence that human mast cells contribute to the newlinepathophysiology of asthma. Mast cells, but not T cells or eosinophils, localize within the newlinebronchial smooth muscle bundles in patients with asthma but not in normal subjects or newlinethose with eosinophilic bronchitis, a factor likely to be important in determining the newlineasthmatic phenotype. Currently, 8.4% of people in the United States have asthma as newlinecompared with 4.3% of the population worldwide, and both numbers are on the rise. The newlineobjective of the present study was to prepare hydroalcoholic extracts of leaves of newlineJasminum sambac and shoots of Triticum aestivum and study the inhibition of mast cell newlinedegranulation activity of the extract and report the most promising extract through its newlinebioactivity guided result. The plant materials were extracted with solvents of various ratio newline(30:70; 50:50; 70:30 and 100% ethanol) of water: ethanol mixture by cold percolation newlinemethod, followed by a qualitative phytochemical investigation. Extracts were subjected newlineto evaluation of acute toxicity study and assessment of mast cell stabilizing activity newlineagainst compound 48/80 induced degranulation. The promising extract was fractioned by newlineliquid-liquid fractionation and fractions were screened for quantitative estimation of total newlinephenolic and flavonoid contents. Flavonoid/ phenolic compound rich fractions were newlineanalyzed with the spectroscopic method. From the investigation it was found that water: newlineethanol (50:50) extracts of Triticum aestivum and Jasminum sambac showed significant newlinepercentage protection. For the extract of leaves of Jasminum sambac the percentage protection newlinewas found to be 54% and 63% at dose levels of 25 mg/ml and 50 mg/ml respectively. Also, for newlinethe extract of shoots of Triticum