Structural and functional characterization of sialic acid uptake and metabolism in pathogenic bacteria

Abstract

The current study titled Structural and functional characterization of sialic acid uptake newlineand its metabolism in pathogenic bacteria is divided into 5 chapters. newlineChapter-I is an outline and review of literature on sialic acids and their metabolism in newlinepathogenic bacteria and eukaryotes. Chapter II describes the experimental procedures used newlinein this study. Some of these experimental procedures are published in the journal Microbial newlineBiotechnology titled Automation aided optimization of cloning, expression and purification newlineof enzymes of the bacterial sialic acid catabolic and sialylation pathways enzymes for structural newlinestudies . Chapter-III describes the studies related to the structural and functional newlineXXIII newlinecharacterization of SatA from Haemophilus ducreyi. The results of this study were published newlinein the Journal of Biological Chemistry in an article titled Molecular characterization of the newlineinteraction of sialic acid with the periplasmic binding protein from Haemophilus ducreyi . newlineChapter IV describes the studies related to the structural and functional characterization of Nacetyl newlinemannosamine kinases from pathogenic bacteria. The results of this study have been newlinecommunicated and is currently under review. The manuscript is titled To Zinc or not to Zinc newlineStructure and function of N-acetylmannosamine kinases from pathogenic bacteria . Chapter newlineV discusses some future directions. newlineOverall, our research findings on sialic acid binding protein from the ABC transport newlinesystem outlines the molecular level details of these proteins and their binding to sialic acids. newlineAdditionally, studying the structural and functional details of SatA from the ABC transport newlinesystem in comparison to previously studied SiaPs of TRAP transport system will not only newlinedivulge the details of their interactions with substrates, but also delineate the strategies used by newlinethese pathogenic bacteria to survive in the host system. newline