Phytochemical investigation antioxidant activities and hepatoprotective efficacy of indigofera tirunelvelica sanjappa against ccl4 induced wistar albino rats

Abstract

The therapeutic value of Indigofera tirunelvelica Sanjappa (Fabaceae) has been newlinerecognized as a component of traditional medication for the treatment of various human newlineailments. Though this plant is being highly explored, sufficient evidences for the mode of newlineaction of lead molecules present in the plant is yet to be unrevealed. Hence, this present newlinestudy was undertaken for screening the hepatoprotective potentials of whole plant powder newlineof Indigofera tirunelvelica against CCl4 induced hepatotoxicity using in-vitro, in- vivo newlineand in-silico studies. The plant was screened both chemically and biochemically, before newlineconducting in-vitro and in-vivo experiments, to assess the consistency of the results. The newlinesecondary metabolites present in the different extracts of whole plant powder were newlineidentified qualitatively and the important phytochemical compounds were also estimated newlinequantitatively. The free radical scavenging activity was tested through various newlineantioxidant free radical scavenging assays. It was observed that the ethanol extract of newlinewhole plant of I. tirunelvelica (ItW-Et) has significant free radical scavenging activity newlineand then the ItW-Et was taken for further in-vitro and in-vivo analysis. The outcome of newlinein-vitro cytotoxic effect (MTT Assay) is indicated that ItW-Et has excellent cytotoxic newlineeffect towards Hep G2 cell lines. Toxicity study was carried out to identify the toxic newlineeffects of ItW-Et on Wistar Albino Rats and it was evident that the ItW-Et having non newlinetoxic compounds and taken for screening in-vivo hepatoprotective studies. For in-vivo newlinehepatoprotective examination, animals were divided into seven groups. Group I animals newlineacted as normal Rats, Group II Animals were received CCl4 orally, Group III, IV and V newlineAnimals received orally CCl4 and treated with ethanol extract of ItW-Et (100, 200 and newline400 mg/ Kg BW) for 21 days, Group VI animals received CCl4 orally and treated with newlineSilymarin for 21 days.