Mulberry leaf extract mediated green synthesis of silver nanoparticles and study of its antimicrobial activity

Abstract

ABSTRACT newlineThe study on the biosynthesis of nanoparticles (NPs) has been focused due to its extensive biomedical uses and has great importance in nano-biotechnology science. Developing efficient and environmentally friendly methods for NPs synthesis are vital steps in this cutting edge technology. Among the different NPs, silver nanoparticles (AgNPs) have gained much attention for their exceptional antimicrobial properties. The offbeat physicochemical and biological properties of AgNPs create them ideal for all kinds of uses in pharmaceutical, cosmetics, agricultural, food preservation, and public healthcare. Green approaches for NPs synthesis have overwhelmed the drawbacks of conventional physical and chemical synthesis methods which include a long time ranges, high cost, and toxicity. The biomolecules used in such studies can serve as both reducing and as well as stabilizing agents to generate potential biocompatible AgNPs. In this present investigation two varieties of mulberry plant extracts were used for the biosynthesis of AgNPs. In the first phase, AgNPs were synthesized using the plant extract prepared from the leaves of Morus indica L. V1. Preliminarily AgNPs formation was confirmed by visually and laser light using the phenomenon of Tyndall scattering followed by the UV-Visible (UV-Vis) spectroscopy, X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy and high-resolution transmission electron microscopy (HR-TEM) coupled with energy dispersive X-ray (EDX) spectroscopy. UV-Vis spectra displayed a unique surface plasmon resonance (SPR) band at 460 nm confirmed the bioreduction of Ag+ ions to Ag0. HR-TEM micrographs indicated the uniform distribution of AgNPs with an average diameter of ~54 nm. EDX was studied to detect the signals of elemental silver. The negative zeta potential value of and#8722;14 mV ±2.1, suggested the stability of AgNPs. FT-IR spectra revealed that a shift in the carbonyl stretching from 1639 cmand#8722;1 to 1630 cmand#8722;1 is attributed due to the reduction of Ag+ by the biomolecules.