Please use this identifier to cite or link to this item: http://hdl.handle.net/10603/304898
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dc.date.accessioned2020-11-02T06:11:37Z-
dc.date.available2020-11-02T06:11:37Z-
dc.identifier.urihttp://hdl.handle.net/10603/304898-
dc.description.abstractnewline Xylanases are now becoming one of the major industrial enzymes with vast application in the paper and pulp industries. The presnt study was made to isolate a potential xylanase producing microorganism and to explore a xylan rich residue for the production of xylanase and to make the process economically viable. newlineXylan, the major component of hemicellulose, requires the synergistic action of several hemicellulase enzymes for complete hydrolysis to monomer sugars. The principle enzyme in this process is endo-1,4-and#946;-xylanase, which cleaves the glycosidic bonds between xylosides, generating short xylooligosaccharides. The isolation of a suitable microorganism was carried out using standard plate assay, containing xylan as sole carbon source. Eighteen microorganisms were screened on the basis of growth and zone of hydrolysis. On further screening of the cultures screened, X3 was found to produce maximum xylanase with widest zone of lysis. Various xylan rich agro residues such as corn cob, rice husk, rice bran, sugarcane bagasse and wheat bran were tested for the enzyme production. Low cost wheat bran was selected as substrate under both submerged fermentation (SmF) and solid state fermentation (SSF). The isolated strain produced the maximum enzyme under SmF (12.5U/ml) in comparison to SSF(11.8U/g). It was identified as a novel strain of Bacillus licheniformis KJ842626 belonging to family Bacilllaceae. The bacterium isolated from the soil, produced cellulase free xylanase. Conventional fermentation methods were carried out and process parameters were optimized for the maximum enzyme production. newline newline
dc.format.extentv.129p.
dc.languageEnglish
dc.relation
dc.rightsuniversity
dc.titleCellulase Free Xylanase Production by Bacterial Isolate from Different Agro Residues
dc.title.alternative
dc.creator.researcherChaturvedi, Sarika
dc.subject.keywordBiochemical Research Methods
dc.subject.keywordBiology and Biochemistry
dc.subject.keywordLife Sciences
dc.description.note
dc.contributor.guideKhurana S M Paul
dc.publisher.placeGurugram Manesar
dc.publisher.universityAmity University Haryana
dc.publisher.institutionAmity Institute of Biotechnology
dc.date.registered
dc.date.completed2016
dc.date.awarded
dc.format.dimensions
dc.format.accompanyingmaterialDVD
dc.source.universityUniversity
dc.type.degreePh.D.
Appears in Departments:Amity Institute of Biotechnology

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chapter 1.pdf484.8 kBAdobe PDFView/Open
chapter 2.pdf1.89 MBAdobe PDFView/Open
chapter 3.pdf646.82 kBAdobe PDFView/Open
chapter 4.pdf2.39 MBAdobe PDFView/Open
pre.pdf1.33 MBAdobe PDFView/Open
references.pdf685.46 kBAdobe PDFView/Open
summary.pdf314.13 kBAdobe PDFView/Open
title.pdf418.94 kBAdobe PDFView/Open


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