Ameliorative effect of Boerhavia diffusa against Calcineurin Inhibitors CNI induced nephrotoxicity

Abstract

Calcineurin inhibitors CNI have revolutionized the field of transplantation by improving newlinethe graft survival during solid organ transplantation Currently 94 percent of the transplant patients newlineare discharged with CNI based immunosuppressive drug regimen Cyclosporine and tacrolimus newlineare the two drugs under this group which inhibits calcineurin a calcium and calmodulin newlinedependent phosphatase and thus impede the activation of T cells Apart from this salubrious newlineeffect the drug induces nephrotoxicity in both renal and non renal transplant patients which newlinehampers the long term kidney graft and cause additional morbidity During organ transplantation newlineusage of immunosuppressants is inevitable hence prevention of CNI induced nephrotoxicity newlinebecomes preferable and could be achieved by adjuvant therapy Herbal medicines always show newlinepromising effects in alleviating the toxicity induced by allopathic drugs In the present study newlineBoerhavia diffusa a medicinal plant used in Ayurveda and Siddha medicines for its rejunuvating newlineproperty was used to explore the nephroprotective activity against CNI induced nephrotoxicity newlineIn the present study the ethanolic root extract was prepared and the extract was fractionated newlineusing liquid liquid partitioning with various solvents such as hexane chloroform and newlineethylacetate The fractions were quantified for secondary metabolites and screened for in newlinevitro antioxidant activity and cytoprotective activity using MTT assay Among the various newlinefractions tested the ethyl acetate fraction was found to have surplus amount of secondary newlinemetabolite with effective antioxidant activity newline newline newline