In Vivo Studies to Evaluate the Radioprotective Efficacy of Carica Papaya Linn Extract and Diallyl Disulphide in Irradiated Mice

Abstract

newlineThe present study was conducted to understand the effect of diallyl disulphide and newlineCarica papaya (Linn.) to impede the damaging effects of ionizing radiation. The major newlinepart of the damaging effects can be countered by maintaining antioxidant activity and newlineprotecting the cellular contents and DNA integrity. In order to understand these effects, newlinepreliminary in vitro and in vivo studies were conducted. Further, radioadaptive response newlinewith respect to Carica papaya (Linn.) and diallyl disulphide were conducted. newlineFor in vitro studies, the leaves, pulp and seeds of Carica papaya (Linn.) were subjected newlineto different extraction processes using ethanol, methanol and water. The different newlineextracts were subjected to preliminary phytochemical screening and radical scavenging newlineassays. The extract with higher amount of phytochemicals and maximum radical newlinescavenging activity was selected for further studies. The aqueous leaf extract was found newlineto possess maximum phytoconstituents with higher antioxidant potential and hence newlinechosen for further studies. newlineThe in vivo studies were conducted in Swiss albino mice after the approval from the newlineinstitutional animal ethics committee. The mice were divided into irradiated and nonirradiated newlinegroups with respective controls for Carica papaya (Linn.) and diallyl newlinedisulphide. In addition to sublethal dose response, the adaptive and low dose responses newlinewere also studied. The maximum tolerable dose with 50% survival was calculated and newlinethe optimum radioprotective dose for Carica papaya (Linn.) and diallyl disulphide were newlinedetermined.