Studies on The Genoprotective Effects of Septilin and Gallic Acid On Swiss Albino Mice and Evaluation of Their Cytotoxic Effects On MCF 7 Cells

Abstract

Cyclophosphamide (CP) and cisplatin (Csp) are extensively used chemotherapeutic drugs but use of these drugs is limited due to their cytotoxic and immunosuppressive effects on normal cells. Septilin (Spt) is a polyherbomineral drug formulation from the Himalayan Drug Company. Gallic acid (GA) is a natural triphenolic acid and is widely distributed in fruits, plants, vegetables and in their derivatives. The present work was taken up to study the anticlastogenic, antigenotoxic, antioxidant, histoprotective and immunomodulatory effects of Spt and GA against CP and Csp induced damage in Swiss albino mice and to investigate their cytotoxic effects on human breast adenocarcinoma cells. The protective effects of Spt and GA against CP and Csp induced adverse effects in Swiss albino mice and their cytotoxic effects on human breast adenocarcinoma cells were studied by employing standard methods. In our findings, Spt and GA effectively reduced the clastogenic effects induced by CP and Csp in somatic and male germinal cells of mice. Comet assay revealed the potent antigenotoxic effects of Spt and GA against CP and Csp induced DNA strand breaks in bone marrow cells of mice. Spt and GA pre-treatment has shown improvement in the activity of liver superoxide dismutase and reduced glutathione. Spt and GA pre-treatment also exerted protection against CP and Csp induced hepatic and testicular damage. Co-administration of Spt and GA has reduced the inflammation induced by anticancer agents effectively by enhancing the production of antibodies and by stimulating macrophages. Spt and GA enhanced the cytotoxic potential of Csp on cancer cells without inducing any damage to human normal breast epithelial cells. The outcomes of our study highlights the protective role of Spt and GA in abatement of anticancer drugs induced mutagenicity, genotoxicity, oxidative damage, immunosuppression in mice and the cytotoxic potential of Spt and GA on cancerous cells. Hence, it can be suggested that, Spt and GA can be used as adjuvant with chemotherape