COMPARATIVE TOXICITY PROFILING OF SILVER NANOPARTICLES PREPARED FROM SYNTHETIC AND GREEN METHODS

Abstract

newline Background: Silver nanoparticles have anti fungal property and anti microbial property. These newlinenanoparticles used in medical appliance. Silver nanoparticles also used in biosensors and numerous newlineassays as a diagnostic. But Silver nanoparticles produce toxic effects at cellular level. They are newlineCytotoxicity, Genotoxicity, Liver toxicity and Inflammation. The presence of colloidal silver in newlinefood supplement leads to incurable grayish color of the skin (Argyria). The silver nanoparticles newlineprepared by green method are supposed to be non toxic as mentioned in literature due to absence of newlinechemicals. newlineObjectives: prepare silver nanoparticles by green method (using plant extracts) and synthetic (using newlinechemicals) methods, characterize of silver nanoparticles and toxicity studies and anticancer newlineactivities of silver nanoparticles and compare them. newlineMethodology: These bio and chemical synthesized nanoparticles were characterized with the help newlineof UV Vis Spectroscopy, Nanotarc, etc. Further, we compared the toxicities of green and chemical newlineAgNPs against human skin dermal fibroblast (HDFa) and brine shrimp followed by anticancer newlineactivity in NCI-H460 cells and followed sub-chronic studied as per the OECD guidelines and, newlineGenotoxicity studies. newlineResults: The obtained results from Nanotrac and TEM revealed that the synthesized AgNPs newlinepossess spherical shape with a mean diameter at 94 nm for green and 104 nm for chemical method, newlinethe zeta potential values was _12.02 mV for green AgNPs and _10.4 mV for chemical AgNPs. In newlineaddition, FT-IR measurement analysis was conceded out to identify the Ag+ ions reduced from the newlinespecific functional groups on the AgNPs. We observed green AgNPs cause dose-dependent newlinedecrease in cell viability and increase in reactive oxygen species (ROS) generation. Further, we newlineproved to exhibit excellent cytotoxic effect and induction of cellular apoptosis in NCI-H460 cells. newlinexiv newline newlineSub chronic toxicity evaluation at the dose 300 mg/kg/day for 13 weeks (based on the results newlineof acute studies).