Production Characterization and so study the Proficiency of Microbial Surfactants for Biomedical and Pharmaceutical Applications

Abstract

ABSTRACT newlineBiosurfactants are surface active metabolites produced by microorganisms possess the characteristic property of reducing the surface and interfacial tensions. Like synthetic surfactants, the biosurfactants exhibit physicochemical properties and individuality such as foaming. emulsification. de-emulsification, wetting and detergency. They are effective in stabilizing newly created surface and generally used in food, cosmetics and many pharmaceutical industries. The search for biosurfactant producers within halophilic bacteria appears to be particularly hopeful, because the biosurfactants of these organisms may have adaptations that can increase their stability in adverse environments. The scope of the research is to identify a novel biosurfactant from a potential halophilic bacterium, aiming for effective applications in biomedical and pharmaceutical applications. Soil samples were collected from four different sites of halophilic environments includes, shoreline in Manvalakurichy (MK), fishing harbor in Colachel (CL), saltpan in Puthalam (PT) and Manakudi (MN) in Kanyakumari district, Tamil Nadu, India. In the present study, a total of 27 bacterial colonies were isolated from four soil samples and screened for biosurfactant activity by haemolysis, oil spreading and drop collapse test. A total of 14 positive isolates were subjected to biosurfactant production in Mineral salt medium with Fic diesel as carbon source. Biosurfactants of the isolates were extracted by cold acetone precipitation method and used further studies. Biochemical analysis revealed the biosurfactant to be the mixture of carbohydrate, protein and lipids. The biosurfactants exhibited significant antibacterial and antifungal activity against pathogenic microorganisms and considerable antioxidant property. The selected potential isolates such as MK5, PT3, PT5, MN3 and MN8 were identified as Acinetobacter sp., Halobacillus trueperi, Virgibacillus maristnortui, Salinivibrio costicola and Salinivibrio sp. by 16s rRNA sequence analysis. Function