Analysis of Gene Expression During Post Hatch Period Under Different Feed Regimes in Naked Neck Broiler Post Hatch

Abstract

IGFs are involved in metabolism and body growth by regulating the synthesis and newlinedegradation of glycogen and proteins in animals. In chicken IGF-1 gene, an A570C substitution newlinehas been reported to invariably associated with growth, body composition, skeletal integrity, newlinehormonal and metabolic factors. The present study aimed to detect polymorphism in the newlinerespective region (promoter and 5 UTR) in CSML individuals in two generations using Hinf I newlinePCR-RFLP. An array of economic traits viz. Juvenile body weights, Body conformation traits, newlineCarcass traits and crude protein% in breast muscle were recorded on birds of different genotypes newlineof IGF-1. Expression analysis of various growth related genes i.e. IGF-1, IGF-2, IGF-1 receptor, newlineGrowth hormone receptor, IGFBP-2, Ghrelin, Myostatin and TGF-and#946;2 were also carried out using newlinereal time PCR in liver and breast muscle samples of birds of each of IGF-1 genotype at 45 days of newlineage. Genomic DNA has been isolated from blood using phenol choloroform extraction method. A newlinetotal of 100 and 96 birds were screened for estimating gene and genotypic frequencies in two newlinesuccessive generations. PCR amplified fragment of 813bp was digested with Hinf I. The Hinf I newlinedigestion revealed patterns viz. AA (378, 244 and 191bp), BB (622 and 191bp) and AB ( 622, newline378, 244 and 191bp) in both the generations. The overall frequency of AA, AB and BB genotypes newlinewere 0.74, 0.23 and 0.03; in generation I these were 0.70, 0.27 and 0.03; and in generation II 0.78, newline0.18 and 0.04, respectively. The frequency of allele A and B in generation I was 0.83 and 0.17 newlineand in II generation 0.87 and 0.13, respectively, with an overall frequency of A and B alleles as newline0.85 and 0.15, respectively. Significant differences among IGF-1 genotypes were observed on newlinebody weights at III, IV and V weeks of age in first generation and on body weight at V and VI newlineweeks of age in II generation. IGF-1 AA and IGF-1 AB genotype exhibited higher body weights newlinethan IGF-1 BB genotype birds. Significant effect of IGF-1 genotypes on conformation traits