Design and characterization of artificial receptors for certain pt amino acids and their derivatives based on harmaceutically significanmolecular imprinting technology

Abstract

Biological receptors generated by natural evolution have the capacity for specifically recognizing target molecules from real matrices. Evolution has provided biology with many intriguing examples of molecular recognition, including those involved in interactions between a ligand and a receptor (such as substrate and enzyme, antigen and antibody), and in transport and signal transduction processes. Studies of these molecules have been dependent on our ability to selectively capture these molecules from complex biological mixtures. This tool will remain instrumental in virtually all aspects of molecular biology research. Antibodies have been widely used for selective molecular recognition, however; antibodies exhibit characteristics that limit their applications. These proteins are large complex molecules that need to be stored carefully. An ideal molecular recognition agent should have high specificity and be composed of a stable, robust, nonbiological material. Molecular imprinting is one of few general, nonbiological methods for creating molecular receptors. The nonbiological approach of molecular imprinting creates populations of specific recognition sites in synthetic network polymers. Polymerization of monomers in the presence of target molecule to imprint the structural information is known as molecular imprinting of polymers (MIP). This is a scientific field which is rapidly gaining significance for a wide range of applications in chemistry, biotechnology and pharmaceutical research. Due to their analytically useful properties, such as selectivity, shelf stability, robustness and reusability, MIP offers potential for the synthesis of artificial recognition material and they are being proposed for the development of novel biorecognition elements. A new era has begun by a synergistic merging of synthetic polymers (MIPs) with biomedicine replacing biosensors.