Production, purification, characterization of bacterial lipase and its exploitation in detergent industry

Abstract

Lipases triacylglycerol hydrolases are an important group of hydrolases, having immense industrial applications in food, dairy, detergent and pharmaceutical sectors Among hydrolases, lipases have gained special attention over few years owning to their ability to work in presence of extreme temperature, pH, organic solvents under non aqueous conditions with chemo, region , and enantio selectivity So by keeping in view the immense applications of lipase, the present work has been focused on production, characterization and purification of bacterial lipase and its evaluation as a potential detergent additive newline The bacterial strain, Staphylococcus arlettae JPBW 1 MTCC5589 was isolated from a rock salt mine Darang HP, India was identified as lipase producer Higher lipase yield was observed when cultured in LB media pH 80 supplemented with soybean oil 12 percent using 10 percent inoculum for 3 h at 37 °C under submerged conditions Partially purified lipase 60 percent Ammonium sulphate was found to be active over a broad range of temperature 30 90 °C, pH 70 120 and NaCl concentration 0 20 percent A modeling integrated optimization has been performed to model and optimize the lipase production through Response surface methodology RSM integrated Genetic algorithm GA For building the RSM model, three level five factorial central composite design was utilized by considering the individual and interaction effects of submerged fermentation variables on lipase production