Estimation of Some Phytoconstituents and Synthetic Drugs with Simultaneous Separation of Their Degraded Compounds

Abstract

A precise, specific and selective analytical method was developed for the newlinequantitative determination of the Tapentadol hydrochloride, Berberine chloride, newlineNicotine hydrogen tartrate, Paliperidone and Orlistat in presence of their newlinedegraded compounds. During the study drug was exposed to the different force newlinedegradation conditions like acid, base, peroxide, dry heat and thermal wet. newlineForce degraded samples of the drug under the different conditions was newlinequantitatively determined by using HPLC method. HPLC of make Agilent LC newline1120 was thought the study. Mobile phase, diluent, column was selected and newlinemodified so that drug can be quantitatively determined without the interference newlineof impurity and excipients. newlineFor Tapentadol hydrochloride column Phenomenex C18 (150 mm × 4.6 newlinemm, 5 and#956;m) and mobile phase Acetonitrile: Phosphate buffer 0.05M (pH 3.2) newline40:60v/v was used. Hypersil BDS C18 (150 mm × 4.6 mm, 5 and#956;m) column and newlineAcetonitrile: Phosphate buffer 0.05M (pH3) 25: 75 v/v as a mobile phase newlinewas used for Berberine chloride. Quantitative separation of the Nicotine newlinehydrogen tartrate was conducted by using column Inertsil ODS (3V) C18 (250 newlinemm × 4.5 mm, 5 and#956;m) and mobile phase Methanol: Buffer (25:75 v/v). For newlinePaliperidone Hypersil BDS C18 column (250 mm × 4.6 mm, 5 and#956;m) column newlinewas used with eluent Acetonitrile: phosphate buffer 0.05M (35: 65 v/v). newlineWhile for Orlistat Hypersil BDS C18 column (250 mm × 4.6 mm, 5 and#956;m) and newlinemobile phase Phosphate buffer pH 3: Methanol (70: 30 v/v) was selected. newlineHPLC methods were validated on the basis of different validation newlineguidelines parameters to conform the validity of method. newline