Genetic studies of Vigna mungo x V umbellata derivatives for disease resistance and other economic traits through morphometric and molecular markers

Abstract

The present investigation entitled, Genetic studies of Vigna mungo x V. umbellata derivatives for disease resistance and other economic traits through morphometric and molecular markers was undertaken to study nature of variation and association among seed yield and related traits in Vigna mungo x V. umbellata derived advanced lines, to identify the alien chromatin in these derivatives using PCR based markers and to isolate the superior derivatives having disease resistance and/or other desirable attributes. The experimental material consisting of 61 Recombinant Inbred Lines (RILs) (F7 and F8 generations) of Vigna mungo x V. umbellata along with three standard check varieties of black gram namely, PDU-1, UG-218 and Palampur 93 was raised in and#61537;-Design with three replications and data was recorded on seed yield per plant and various other morphometric traits, crude protein and reaction to various diseases under natural epiphytotic conditions. Wide genetic diversity among RILs was confirmed by analysis of variance, D2 statistics, polymorphism, AMOVA, Principal Coordinate Analysis (PCoA), Neighbor-joining tree, Jaccard s coefficient of similarity and genetic parameters. Novel RAPD and ISSR bands appeared in the RILs indicating the occurrence of genetic changes in the genome of the progenies. Presence of 1400 bp band generated using ISSR 808 primer and gene flow estimates between ricebean and RILs confirmed alien introgression. Pods per plant was found to be the best selection indices for increasing seed yield per plant. Highest indirect effect on seed yield via pod per plant was observed for biological yield per plant, plant height, harvest index and 100-seed weight. Sixteen RILs were found to be superior for seed yield per plant. Seven RILs were found to be resistant for Cercospora leaf spots, 8 RILs highly resistant to anthracnose leaf spots and 1 RIL resistant to powdery mildew. Twenty three lines were found to be significantly superior for crude protein. Palampur 93 x BRS 2 (257-2-L-12-1) followed by Palampur 93 x BRS 2 (257-2-L-2-5), Palampur 93 x Naini (224-2L-5-3) were found to have significantly higher levels of crude protein accompanied with significantly higher yields. newline