Please use this identifier to cite or link to this item: http://hdl.handle.net/10603/2687
Full metadata record
DC FieldValueLanguage
dc.date.accessioned2011-09-08T07:16:30Z-
dc.date.available2011-09-08T07:16:30Z-
dc.date.issued2011-09-08-
dc.identifier.urihttp://hdl.handle.net/10603/2687-
dc.description.abstractSimple sequence repeats (SSRs) are a class of DNA sequences consisting of simple motif that are tandemly repeated at a locus. They are omnipresent in prokaryotes and eukaryotes, even in the smallest bacterial genomes, and are found anywhere in the genome in both protein-encoding and noncoding regions. In present study the whole genome sequences of 10 chromosomes and 7 plasmids, Genes Distributed by COGs, Pathogenicity islands, Pseudogenes, Plasmid virulence genes and chromosome virulence genes were downloaded in FASTA format from ftp://ncbi.nlm.nih.gov/genbank/genomes/ and SHIBASE for identifying abundance, distribution, composition of SSRs and determine difference between the observed and the expected tandem repeats in genome of the organisms. in this study. VNTR Loci and 1 MLVA locus have been selected for Molecular typing of Shigella flexneri 2a. The result obtained in the present study show that: (i) tandem repeats are widely distributed throughout the genomes (ii) SSRs are differentially distributed among coding and noncoding regions in investigated genomes. (iii) total frequency of SSRs innoncoding regions are higher than coding regions. (iv) in all investigated chromosomes ratio of Trinucleotide SSRs are much higher than randomized genome and Di nucleotide SSRs are lower.(v) Ratio of mononucleotide SSRs in real genome is higher than randomized genome in E.coli K12 , Sh.f 301 , S.saprophyticus , while it is lower in Sh.f 2457T, Sh.sonnei and M.tuberculosis and it is approximately same in M.leprae. (vi) Frequency of codon repetitions vary considerably depending on the type of encoded amino acid. (vii) SSR in Shigella pathogenesity islands are overrepresented rather than other gene clusters of Shigella. (viii) Amino acid repeats pattern and codon repeats frequency in Shigella islands is different with other gene clusters (ix) SSR is overrepresented in ipaH genes, mxi, spa, osp genes particularly plasmid ipaH genes.en_US
dc.format.extentiix, 203p.en_US
dc.languageEnglishen_US
dc.rightsuniversityen_US
dc.titleStudy on Shigella virulence genes and development of molecular diagnostic toolsen_US
dc.creator.researcherHosseini, Ashrafen_US
dc.subject.keywordShigellosis, HIV infection, Drugs, GenBanken_US
dc.description.noteAbstract includes, References p.175-203en_US
dc.contributor.guideKhandekar, P Sen_US
dc.contributor.guideChopade, B Aen_US
dc.publisher.placePuneen_US
dc.publisher.universityUniversity of Puneen_US
dc.publisher.institutionDepartment of Biotechnologyen_US
dc.date.registered0en_US
dc.date.completedJuly 2008en_US
dc.date.awarded2008en_US
dc.format.accompanyingmaterialDVDen_US
dc.type.degreePh.D.en_US
dc.source.inflibnetINFLIBNETen_US
Appears in Departments:Department of Biotechnology

Files in This Item:
File Description SizeFormat 
01_title.pdfAttached File41.44 kBAdobe PDFView/Open
02_contents.pdf64.15 kBAdobe PDFView/Open
03_acknowledgements.pdf13.42 kBAdobe PDFView/Open
04_abstract.pdf10.23 kBAdobe PDFView/Open
05_list of abbreviations.pdf18.21 kBAdobe PDFView/Open
06_list of tables.pdf79.13 kBAdobe PDFView/Open
07_list of figures.pdf81.14 kBAdobe PDFView/Open
08_chapter 1.pdf628.62 kBAdobe PDFView/Open
09_chapter 2.pdf1.57 MBAdobe PDFView/Open
10_chapter 3.pdf1.15 MBAdobe PDFView/Open
11_chapter 4.pdf452.8 kBAdobe PDFView/Open
12_chapter 5.pdf234.49 kBAdobe PDFView/Open
13_references.pdf289.07 kBAdobe PDFView/Open
14_appendix.pdf635.16 kBAdobe PDFView/Open


Items in Shodhganga are licensed under Creative Commons Licence Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0).

Altmetric Badge: