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http://hdl.handle.net/10603/2681
Title: | Antioxidant, antimicrobial and chemopreventive efficacy of Raphanus sativus |
Researcher: | Syed Sultan Beevi, S |
Guide(s): | Lakshmi Narasu, M |
Keywords: | Plants, Therapeutic agents, Foods, Cruciferous plants |
Upload Date: | 7-Sep-2011 |
University: | Jawaharlal Nehru Technological University |
Completed Date: | February 2010 |
Abstract: | Raphanus sativus, a common cruciferous vegetable has been attributed to possess a number of pharmacological and therapeutic properties. Water, methanol, acetone, ethyl acetate, chloroform and hexane extracts derived from root, stem and leaves of R. sativus were evaluated for the presence of phytochemicals such as isothiocyanates (ITCs) and polyphenolics. These extracts were checked for antioxidant and radical scavenging activity, antimicrobial activity, protective effect against oxidative damage induced by oxidants and anti-proliferative activity towards human cancer cell lines such as HeLa, A549, MCF-7 and PC-3 cells. Isothiocyanates (ITCs) were detected in considerable amount in root, stem and leaves of R. sativus. However, root extracts contained highest levels of ITCs, as compared to leaves and stem extracts. Highest amount of ITCs was extracted from root, when hexane was used as an extraction solvent. However, polar solvents were effective in recovering considerable amount of polyphenolics from R. sativus. R. sativus had total polyphenolic content, which was comparable to traditional rich source such as green and black tea. HPLC analysis indicated the presence of catechin, protocatechuic acid,syringic acid, vanillic acid, ferulic acid, sinapic acid, o-coumaric acid, myricetin and quercetin in R. sativus. Among different parts of R. sativus, leaves contained highest amount of phenolics and showed strongest antioxidant and radical scavenging activity. Of the different extraction solvents used, methanolic extract showed effective reductive capacity and significantly inhibited linoleic acid peroxidation, displayed metal chelating activity and effectively scavenged free radicals such as DPPH radicals, superoxide radicals, hydrogen peroxide and nitric oxide radicals respectively. All extracts except water extract of root, stem and leaves had a significant broad spectrum inhibitory activity. Ethyl acetate extract of root had potent antibacterial activity with significant inhibition, comparable to that of standard antibiotics against pathogenic bacteria. This was followed by ethyl acetate extract of leaves and stem. Further, ethyl acetate extract of different parts of R. sativus retained their antibacterial activity after heat treatment at 100°C for 30 min and their antibacterial activity was enhanced when pH was maintained in the acidic range, demonstrating their thermal stability and acid tolerance properties. Compositional analysis of ethyl acetate extract of root by GC-MS revealed the presence of polyunsaturated fatty acids (PUFAs), ITCs such as 4-(methylthio)-3-butenyl isothiocyanate (MTBITC) and 4-(methylthio)-3-butyl isothiocyanate (erucin), alkanes, eugenol and methyl cholesterol. Significant antibacterial activity of ethyl acetate extract of R. sativus root could be attributed to complex mixture of phytochemicals present in it R. sativus extracts per se showed no cytotoxicity and genotoxicity to lymphocytes. |
Pagination: | xvi, 204p. |
URI: | http://hdl.handle.net/10603/2681 |
Appears in Departments: | Faculty of Biotechnology |
Files in This Item:
File | Description | Size | Format | |
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01_title.pdf | Attached File | 78.27 kB | Adobe PDF | View/Open |
02_declaration.pdf | 70.83 kB | Adobe PDF | View/Open | |
03_acknowledgements.pdf | 73.29 kB | Adobe PDF | View/Open | |
04_abstract.pdf | 88.09 kB | Adobe PDF | View/Open | |
05_table of contents.pdf | 79.89 kB | Adobe PDF | View/Open | |
06_list of figures.pdf | 75.88 kB | Adobe PDF | View/Open | |
07_list of tables.pdf | 77.54 kB | Adobe PDF | View/Open | |
08_glossary.pdf | 68.14 kB | Adobe PDF | View/Open | |
09_chapter 1.pdf | 127.83 kB | Adobe PDF | View/Open | |
10_chapter 2.pdf | 369.19 kB | Adobe PDF | View/Open | |
11_chapter 3.pdf | 182.56 kB | Adobe PDF | View/Open | |
12_chapter 4.pdf | 857.17 kB | Adobe PDF | View/Open | |
13_chapter 5.pdf | 105.64 kB | Adobe PDF | View/Open | |
14_chapter 6.pdf | 256.49 kB | Adobe PDF | View/Open |
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