Study of Dielectric Relaxation and Thermo physical Behaviour of some Biomolecules Using Spectroscopic Technique

Abstract

Dielectric relaxation spectroscopy in liquids is widely used for determination of the newlinestructure and associated liquid properties which are helpful to study the molecular newlineinteractions. In this work , structural dynamics of aqueous amino acids have been studied newlinethrough relaxation processes. L- Glycin and L-Alanine amino acids have been used in the newlinewhole concentration range for the study. Time domain spectroscopy working in the newlinefrequency region of 10MHz to 30GHz at room temperature has been employed for the newlinepurpose. Two relaxation processes, low/slow and high/fast has been observed for aqueous newlineL-Glycine whereas, two relaxation modes has been detected for aqueous L-Alanine. newlineDielectric parameters such as static dielectric constant (and#949;and#8320;) , high frequency limiting newlinedielectric constant (and#949;and#8734;) and average relaxation time (and#964;and#8320;),relaxation streangth (and#916;and#949;) have newlinebeen calculated for the amino acids for it s different concentration . Molecular interaction newlinebetween solute and solvent at different molar concentration and temperature gives newlinevaluable information about hydration number(NHyd),Effective hydration number(Zib), newlineCorrelation factor(gb),Effective dipole moment (and#956;eff) Thermo dynamical parameters such newlineas free energy of activation (and#916;F), entropy of activation ( and#916;S) and enthalpy of activation newline(and#916;H)have also been studied for both low and high processes in the studied systems. newlineAverage entropy of activation and enthalpy of activation observed in case of glycin for newlineboth low and high processes is higher than the values found in case of alanine. Free newlineenergy of activation in case of glycin for high process is slightly greater than that newlineobserved in case of alanine, whereas free energy of activation observed in case of low newlineprocesses was found to be slightly greater in alanine than glycin .this will give us new newlineinsights about molecular dynamics in bio molecules newlineWater is a highly versatile component at the interface of biomolecular complexes. newlineIt can act both as a hydrogen bond donor and acceptor, imposing few steric constraints on newlinebond formation a