Please use this identifier to cite or link to this item: http://hdl.handle.net/10603/255894
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dc.coverage.spatialBiotechnology
dc.date.accessioned2019-08-27T10:39:20Z-
dc.date.available2019-08-27T10:39:20Z-
dc.identifier.urihttp://hdl.handle.net/10603/255894-
dc.description.abstractSaussurea lappa has innumerable pharmacological properties and sesquiterpene lactones are considered as the principle components confering these properties to the plant. Costunolide is among the chief sesquiterpene lactones occurring in the plant. De novo transcriptome sequencing of leaf tissue using Illumina HiSeq 2000 sequencing platform yielded 62,039,614 high quality paired end raw reads. These reads were assembled using Trinity assembler and 122,434 transcripts were obtained which were further clustered into 110,283 unigenes using CD-HIT. The transcripts were functionally annotated using BLAST similarity search tool against several databases. The transcripts showed significant homology with already known genes. Further, about newline9,536 GO terms were assigned to the transcripts, which were classified into three categories viz. molecular function, biological process and cellular component category. The SSR markers were discovered in the transcripts using MISA perl script and 9.05% transcripts were found to contain SSRs. In-silico analysis enabled the discovery of mevalonate pathway and costunolide biosynthetic genes. Also, genes involved in flavonoid and alkaloid biosynthesis were obtained in the dataset. Further qRT-PCR based relative expression analysis was conducted and higher expression of genes of costunolide biosynthesis was observed in leaf tissue as compared to root tissue. The molecular cloning of Mevalonate kinase, Phosphomevalonate kinase, Diphosphomevalonate decarboxylase and Germacrene A Synthase was also carried out. This is the pioneer study pertaining to the functional genomics studies in S. lappa.
dc.format.extent123p.
dc.languageEnglish
dc.relation-
dc.rightsuniversity
dc.titleFunctional genomics studies of costunolide biosynthesis in Saussurea lappa
dc.title.alternative-
dc.creator.researcherBains, Savita
dc.subject.keywordAsteraceae
dc.subject.keywordCostunolide
dc.subject.keywordLife Sciences,Microbiology,Biotechnology and Applied Microbiology
dc.subject.keywordSaussurea lappa
dc.subject.keywordSesquiterpene lactone
dc.subject.keywordTranscriptome
dc.description.noteSummary p.101-102, References p.103-123, Appendices p.i-xlvii and Publications
dc.contributor.guideKashmir Singh
dc.publisher.placeChandigarh
dc.publisher.universityPanjab University
dc.publisher.institutionDepartment of Biotechnology
dc.date.registeredn.d.
dc.date.completed2019
dc.date.awardedn.d.
dc.format.dimensions-
dc.format.accompanyingmaterialCD
dc.source.universityUniversity
dc.type.degreePh.D.
Appears in Departments:Department of Biotechnology

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01_title.pdfAttached File25.24 kBAdobe PDFView/Open
02_certificate.pdf745.38 kBAdobe PDFView/Open
03_acknowledgment.pdf72.47 kBAdobe PDFView/Open
04_contents.pdf7.19 kBAdobe PDFView/Open
05_abbreviations.pdf114.35 kBAdobe PDFView/Open
06_chapter1.pdf572.9 kBAdobe PDFView/Open
07_chapter2.pdf547.79 kBAdobe PDFView/Open
08_chapter3.pdf549.15 kBAdobe PDFView/Open
09_chapter4 .pdf3.92 MBAdobe PDFView/Open
10_chapter5.pdf391.35 kBAdobe PDFView/Open
11_summary.pdf200.5 kBAdobe PDFView/Open
12_references.pdf530.48 kBAdobe PDFView/Open
13_appendices.pdf904.1 kBAdobe PDFView/Open
14_publications.pdf134.84 kBAdobe PDFView/Open


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