DNA Barcoding And Phylogeny Based Comparative Evaluation Of Anti Cancer Properties Of Caulerpa J V Lamouroux spp From Indian Coasts

Abstract

iv newlineABSTRACT newlineDNA barcoding and phylogeny based comparative evaluation of anti-cancer newlineproperties of Caulerpa (J V Lamouroux) spp. from Indian coasts newlineName of Student Richa Mehra newlineRegistration Number CUPB/MPHIL-PHD/SBAS/BIO/2012-13/05 newlineDegree for which submitted Doctorate of Philosophy newlineName of Supervisor Dr. Felix Bast newlineName of Co-Supervisor Dr. Sandeep Singh newlineDepartment Centre for Biosciences newlineSchool of Studies School of Basic and Applied Sciences newlineKeywords Caulerpa, barcoding, cancer, phylogeny, anticancer newlineA total of 15 Caulerpa samples were collected from Indian coasts and newlineidentified based on morphological and molecular data inferred from ITS, 18S, tufA newlineand rbcL. Seven different species viz. C. scalpelliformis, C. racemosa, C. newlinesertularioides, C. verticillata, C. taxifolia, and C. corynephora; and their geographical newlineisolates were identified. Barcode data for these species was generated using newlineaforementioned molecular markers and used for phylogenetic assessment. newlinePhylogenetic trees using Bayesian inference (BI) and Maximum Likelihood (ML) newlinefunction were generated for each molecular marker. tufA was found to be most newlinesuitable marker for the genus Caulerpa, resolving the species into 17 different newlinelineages, with 15 corresponding to already known sections and 2 new lineages. newlineBesides, a database named DbIndAlgae of Indian algae was generated and all the newlinemorphological as well as molecular data generated in this study is uploaded on the newlinedatabase. In addition, the phycochemical analysis revealed the presence of newlinealkaloids, terpenoids, steroids, tannins, saponins, flavonoids, and phenols in newlinedifferent Caulerpa species. The selective cytotoxicity of methanolic extracts of newlineCaulerpa (CMEs) was evaluated on MDA-MB-231, T47-D and H1299 cells, and the newlineresults revealed significant cytotoxicity of all species. C. racemosa KNY-254 and C. newlinetaxifolia TEN-158 were found to be most potent on MDA-MB-231 cells with IC50 newlinevalue of 0.226 ± 0.004 and 0.246 ± 0.009 and#956;g/and#956;L. The mitochondrial membrane newlineperturbation was revealed by JC-1 and apoptotic cell death