Biochemical Studies on the effect of Melia Azedarach and Piper Longum in hepatotoxic liver diseases

Abstract

INTRODUCTION: The liver is a vital organ present in vertebrates and other animals. The liver is both the largest gland and largest internal organ in the human body, plays a major role in metabolism and has a number of functions in the body, including glycogen storage, decomposition of red blood cells, plasma protein synthesis, and detoxification. AIM: The present investigation was aimed to determine the hepatoprotective effect of the iherbal extract (BHE), made up of equal proportions of Melia azedarach leaves and Piper longum seeds in experimental animals with CCl4 as hepatotoxic model. The hepatoprotective effect of the Biherbal extract (BHE) was also compared with biological efficacy of individual plant preparations. Objective: newlineThe study was carried out in the following stages. 1. Preparation of the bi herbal ethanolic extract (BHE) of the leaves of Melia azedarach and seeds of Piper longum and their individual ethanolic extracts. 2. To assess the acute and chronic toxicity of BHE. 3. To determine the effective dose of the test drug which gives optimum therapeutic effect. 4. To compare the hepatoprotective effect of BHE with the standard drug Silymarin 5. To evaluate a tentative mechanism of action of the drug. CONCLUSION: In conclusion, the BHE afforded protection from CCl4 induced liver damage, significantly when compared to that of the individual preparations. The beneficial effects of BHE were found comparable to those of silymarin. By trapping oxygen related free radicals, the extract could hinder their interaction with polyunsaturated fatty acids and abolish the enhancement of lipid peroxidative processes. The effect may be due to the presence of flavonoids and phenolic compounds which are strong antioxidants Antioxidant principles from herbal resources are multifaceted in their effects and provide enormous scope in correcting the imbalance through their consumption. It has promising therapeutic application in future to combat the liver related problems associated with free radical formation. newline newline