Pharmacological Screening of Memecylon Sisparense Gamble for its Anti_Microbial_ Anti_Oxidant And Anti_Cancer Activities

Abstract

Memecylon sisparense Gamble (MSG) belongs to melastomataceae family, having wide range of pharmacological activities like antioxidant, hepatoprotective, anti-inflammatory, anti-diabetic etc. For the first time, this study is aimed towards identification of biologically active compounds in MSG leaf ethyl acetate extract (MSGLEAE) by GC-MS analysis along with molecular docking studies for confirming the biological activity of identified compounds. The antioxidant, anticancer potential of the MSGLEAE was studied by in vitro and in vivo models. newlineOut of 41 compounds identified, 20 were found having biological activities like nephroprotective, anti-cancer, antioxidant, antibiotic, hepatoprotective, inhibition of uric acid production etc. The identified compounds were docked against the respective protein structures for cardioprotective, nephroprotective, anticancer activity. newlineMSGLEAE has shown good antioxidant activity along with anti-bacterial activity having highest zone of inhibition on Staphylococcus aureus followed by S. epidermidis then Pseudomonas aeuriginosa, Eschericia coli followed by Bacillus subtilis, B. cereus with lowest zone of inhibition respectively. newlineSwiss albino male mice were treated with MSGLEAE (250, 500 mg/kg, p.o) for nine consecutive days against doxorubicin (DOX) (15 mg/kg, i.p) and cisplatin (12 mg/kg, i.p) on seventh day and evaluated for cardioprotective, nephroprotective activity. newlineIn Doxorubicin induced cardiotoxicity, the changes in heart tissue were assessed from ECG recording in which MSGLEAE decreased the ST segment which was elevated in DOX treated animals. In biochemical estimation of CK-MB, LDH levels found to be increased significantly in DOX group by comparing to control group. Cisplatin induced nephrotoxicity is identified by an increase in serum blood urea nitrogen and creatinine which was decreased in the extract pre-treated groups.