Isolation identification and antibiogram of nonfermenting gram negative bacilli from various clinical samples

Abstract

newline Non-fermenting Gram negative bacilli (NFGNB) NFGNB isolated from various clinical samples of urine, blood, pus, sputum, endotracheal secretions, tracheal aspirate and body fluids. newlineA total of 2261 clinical samples were processed and 365 NFGNB were obtained accounting for their prevalence of 16.1%. Out of these, maximum isolates were obtained from pus samples (24.9%), followed by urine (23.2%), endotracheal secretions (17.8%) and rest from other samples. P. aeruginosa was the commonest NFGNB isolated in this study accounting for 52.6% of total NFGNB isolates. A. baumannii was the second common NFGNB isolated (31.7%). NFGNB isolates obtained with a lesser frequency were- P. fluorescens, P. stutzeri, P. putida, A. lwoffii, Stenotrophomonas maltophilia, Alcaligenes faecalis, Sphingomonas paucimobilis, Burkholderia cepacia, Achromobacter xylosoxidans and Brevundimonas diminuta. P. aeruginosa isolates showed 100% sensitivity to polymyxin B and colistin. 71.4% sensitivity was reported towards imipenem whereas very less sensitivity was reported towards cephalosporins and other antibiotics. Most of the newlineA. baumannii isolates showed multidrug resistance pattern. However, 96.5% sensitivity was recorded for polymyxin B and 97.4% for colistin respectively. newlineIn P. aeruginosa, ESBL production was seen in 17.7% isolates and MBL production was observed in 21.8% isolates. In A. baumannii, ESBL production was seen in 27.5% isolates and MBL production was observed in 44.8% isolates. Out of twenty phenotypically identified MBL positive isolates of P. aeruginosa and A. baumannii, blaVIM gene was detected in four isolates of P. aeruginosa and twelve isolates of A. baumannii. newline