Effect of pre freeze supplementation of novel ant oxidation L Ergothioneine on chromatin integrity and functional parameters of frozen human sperm

Abstract

The objective of the study was to assess the conservative effects of antioxidant L Ergothioneine on the post thaw chromatin integrity and functional parameters of human spermatozoa 400 normozoospermic semen samples were cryopreserved with and without supplementation of EGT Clinically relevant tests such as Semen analysis according to WHO 2010 Sperm chromatin integrity and Lipid peroxidation status assessment were performed in both fresh and frozen thawed samples It was seen that with the increase in ROS production due to cryopreservation there is a significant decrease in the post thaw sperm parameters and sperm chromatin integrity and the extent of which was reduced on addition of EGT The increasing ROS levels correlated directly with DNA damage Hence the study provides a basal proof that EGT acts against the ROS and conserves the spermatozoa functionality newline