Studies on multi therapeutic potential of native carrageenan from red seaweed Kappaphycus alvarezii Doty 1996 in comparison with commercial carrageenan

Abstract

Sulphated polysaccharides from seaweeds are one of the most beneficial newlinecompounds with the wide range of pharmaceutical and biomedical applications. In newlinethe present study, red seaweed Kappaphycus alvarezii was subjected to extraction newlinefor the isolation of its native carrageenan polysaccharide. The percentage yield of newlinenative carrageenan was found to be 33.09%. Physico-chemical, phytochemical and newlinestructural analysis of native carrageenan was determined and compared to those of newlinecommercial carrageenan. The results revealed that both carrageenans were almost newlinesimilar in all the characteristic properties. Therefore, further they were chosen for in newlinevitro antioxidant, anticancer, antidiabetic, anti-inflammatory and anticoagulation newlinestudies. The results on the antioxidant activities exhibited that native and newlinecommercial carrageenans rendered significant total antioxidant, hydroxyl radical newlinescavenging, nitric oxide radical scavenging, DPPH radical scavenging and reducing newlinepower assays in comparison with standard BHA. Anticancer activities of both newlinecarrageenans showed better inhibition on the growth of breast (MCF7), colon (HT- newline29), liver (Hep G2) and osteosarcoma (MG63) cell lines at the maximum newlineconcentration of 150and#956;g/ml, when compared to the standard drug cyclophosphamide. newlineThe results on in vitro anti-inflammatory and antidiabetic assays exhibited that, both newlinecarrageenans (100-500and#956;g/ml) significantly increased the inhibition percentage of newlineheat induced protein denaturation (which is the hall mark of inflammation) and - newlineglycosidase enzyme activity, when compared with the standard drugs diclofenac newlinesodium and acarbose. newline