Antioxidant and antitumorigenic efficacy of methanolic extracts of gloriosa superba and silver nanoparticles of methanolic extracts of gloriosa superba to dla tumor cells

Abstract

The experiments were carried out as in vitro and in vivo studies to evaluate the antioxidative and antitumorigenic efficacy of methanolic extract of Gloriosa superba seed tuber and leaves MGsSTL and silver nanoparticles of methanolic extract of Gloriosa superba seed tuber and leaves AgMGsSTL Characterization of MGsSTL and AgMGsSTL by phytochemical screening HPTLC UVVisibleSpectroscopy FTIR SEM with EDX TEM DLS ZetaPotential and XRD Antioxidative role of MGsSTL and AgMGsSTL were evaluated by assessing their scavenging activity towards radicals In vitro antitumorigenic activity of MGsSTL and AgMGsSTL were evaluated by MTT and tryphan blue assay to Dalton lymphoma ascites DLA tumor cells Apoptotic activity of AgMGsSTL was also carried out by flow cytometry In vivo antioxidative and antitumorigenic potential of MGsSTL and AgMGsSTL was evaluated by the assessment of enzymic antioxidants, levels of non enzymic antioxidants and lipid peroxide levels in DLA induced mice The phytochemical analysis of MGsSTL showed the presence of Carbohydrates Alkaloids Glycosides Flavonoids Tannins Steroids Terpenoids and Phenolics HPTLC studies confirmed the presence of colchicine colchicines derivatives and unknown alkaloids in MGsSTL Silver nanoparticles synthesized by green route was characterized using standard techniques like UVVis spectroscopy FTIR SEM EDX TEM DLS Zeta potential and XRD have a great potential in biomedical applications In vitro antioxidant potential of AgMGsS was found to be more than that of all other extracts The in vitro antitumorigenic effect of AgMGsSTL showed more toxicity towards DLA cells than that of MGsSTL The apoptotic effect of AgMGsSTL to DLA tumor cells confirmed by flow cytometric analysis AgMGsSTL were effective in inhibiting the tumor growth in DLA induced Swiss albino mice when compared to MGsSTL Both in vitro and in vivo biochemical parameters life span and histological studies supported the antioxidant and antitumorigenic properties Our results revealed that AgMGsSTL after clinical trials can