Please use this identifier to cite or link to this item: http://hdl.handle.net/10603/222746
Title: Chromium removal from tannery effluent by microbial biomass
Researcher: Sharma, Indu
Guide(s): Goyal, Dinesh and Das, N.
Keywords: Bacteria
Chromium
Engineering and Technology
FTIR
Kinetic models
Microbial biomass
Tannery
University: Thapar Institute of Engineering and Technology
Completed Date: 2010
Abstract: In the present study bacterial isolates and consortium isolated from tannery effluent (CT) and chrome sludge (CS) were analyzed for Cr(VI) reduction. Molecular identification based on 16S rDNA sequence analysis led to the characterization of isolates as Raoultella sp. (CT4) and Citrobacter sp. (CT5) from tannery effluent and Bacillus cereus (CS7) and Citrobacter freundii (CS8) from chrome sludge. Studies revealed that the bacterial isolate Raoultella sp. (CT4) completely transformed Cr(VI) to Cr(III) whereas Bacillus cereus (CS7), Citrobacter freundii (CS8) and Citrobacter sp. (CT5) showed 94.81%, 95.8% and 95.2% of reduction respectively. Since biowastes from industrial fermentation units can serve as an economical and constant supply of biomass for biosorption of metal ions therefore two types of microbial biomass, which are generated as a byproduct of pharmaceutical fermentation industry involving fermentative production of certain antibiotics by Aspergillus sp. (MB1) and Streptomyces sp. (MB2) were collected from Ranbaxy (fermentation industry) Paonta Sahib, Himachal Pradesh, India and were characterized for physical and chemical parameter such as, pH, moisture, ash content, bulk density, CHN analysis and calorific value. Removal of Cr(III) from aqueous solution and tannery effluent was carried out using microbial biomass (MB1 and MB2) and various parameters including adsorbent dosage (0.25-2%), pH (2-6) and chromium concentration (5-50 mg/L) and contact time were standardized in batch mode and to investigate the mechanism of metal uptake by Fourier transform infrared (FTIR) and X-ray diffraction (XRD) analysis was done. Fourier transform infrared studies with microbial waste biomass revealed the involvement of C=N, C=C, C-H and C-O functional groups in chromium binding. Langmuir and Freundlich adsorption isotherms were predicted from the equilibrium sorption data.
Pagination: 265p.
URI: http://hdl.handle.net/10603/222746
Appears in Departments:Department of Biotechnology

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