Biotransformation: in synthesis of bioactive molecules, 19-HETE and 20-HETE, from Arachidonic acid

Abstract

The major objectives of the thesis were to explore the biotransformation of arachidonic acid for production of 19-HETE and 20-HETE using Candida bombicola. Chapter one presents a general introduction about eicosanoids and biotransformation of arachidonic acid metabolites. 20-hydroeicosatetraenoic acid (20-HETE) and 19- hydroxyeicosatetraenoic acid (19-HETE) are omega and omega–1 hydroxylated products of arachidonic acid which are important in autoregulation of blood pressure, vascular tone and other physiological roles. Different methods were discussed for the production of 20-HETE and 19-HETE. The intermediate form, sophorolipid was discussed in brief. Chapter two describes the fermentation parameters of Candida bombicola (ATCC 22214) for the production of arachidonic acid derived sophorolipids. The derived sophorolipids on acid hydrolysis liberated 19-HETE and 20-HETE. The different experimental techniques and analytical tools were used during the course of the present work are discussed in detail. Chapter three deals with purification of the mono-, di- acetate forms of lactonic and acidic sophorolipids produced by Candida bombicola grown on glucose and long chain fatty acid i.e. arachidonic acid. Chapter four focuses on the continuous transformation of 19-HETE and 20-HETE from arachidonic acid by immobilized Candida bombicola on different biocompatible materials. Chapter five presents the summary of the results obtained and the conclusions drawn are presented in this chapter.