Use of Graphene Based Interfacing Nanoscale Bio Components in Biosensing Device for Common Bacterial Detection

Abstract

In this work, we render a new approach for sensitive detection of pathogenic bacteria based on three different platforms namely a GO PCTE nanosieve platform for gp140MS, GO modified QCM and IR spectroscopy based E. coli detection by applying GO nanofilm over PCTE membrane. An antiand#946;gal antibody was conjugated via linker molecules on GO coated surface cross react with the target antigen in the model assay. However, certain limitations with respect to binding of various proteins as those of E. coli and BSA was revealed since their specific interactions could not be well recorded. Under basic conditions, the frequency of the QCM was inversely proportional to the concentration of the antigen in the given sample. newlineThe fabrication and implementation of a new cell based biosensor encompassing the advantages of IR spectroscopy, with the ability to detect E. coli as well as a broad range of other microorganisms, including viruses have been outlined here. E. coli cells react with anti-and#946;-gal antibody immobilized over GO coated surface show a shift in IR peak from 3373.14 to 3315 cmand#8722;1. In contrast, GO surface coated with anti-Pseudomonas antibody showed no such shift. Thus, the detection of E. coli via. GO nanobiosensor proved to be highly specific and useful for real-time monitoring of bacteria with a detection limit of 100 and#956;g.mL-1 to 10 and#956;g.mL-1. An electrochemical biosensor based on the GO PCTE platform is proposed for rapid and sensitive detection of HIV1 enveloped glycoprotein. An engineered antibody covalently linked with HIV1 CD4 glycoprotein receptor show an exponential decrease in an ionic current recorded as a function of applied voltage in the range of 0.1 2.0v due to the specific interaction of gp140MS with 2Dm2m. Binding of non-specific antigen revealed no change when tested under identical conditions. The receptor-ligand binding was again tested to define the least concentration of target analyte with low detection limit, sensitivity and response time of 8.3 fM, 0.87 mA-mM-1cm-1 and 12 s, respectively. newline