Please use this identifier to cite or link to this item: http://hdl.handle.net/10603/218730
Title: Genomics of Female Flowering ans Seed Yield in Jatropha Curcas L
Researcher: Gangwar, Manali
Guide(s): Jata Shankar and Chauhan, Rajinder Singh
Keywords: Cytokinin
Jatropha biomass
Jatropha flowering
Jatropha sex determination
Monoecious
Yield
University: Jaypee University of Information Technology, Solan
Completed Date: 2018
Abstract: Increased demand of fossil fuels has raised an interest towards culling an alternate and promising renewable source of energy. Jatropha curcas L., a non-edible energy crop of the family euphorbiaceae, has showed potential to provide feedstock for biodiesel due to its high oil content (42%) in the seed. The stumbling roadblock for commercialization of Jatropha based biodiesel production is low seed yield per inflorescence which is governed by low ratio of female to male flowers (1:25-30). Exogenous application of cytokinin was done on floral meristems to increase the female to male flower ratio and seed yield. It increased female flowers (~8-fold) and number of fruits (~3-fold) however, decrease in fruiting and seed size/weight was reduced. Thus, current study was under taken with the following objectives; (1) deciphering molecular cues for floral transitions and female flowering in Jatropha (2) deciphering molecular components of cytokinin on flowering and source-sink relationship of Jatropha. newlineComparative genomic and gene expression profiling was done at floral developmental stages (vegetative to reproductive, differentiation of floral buds into male/female and transition towards female flowering) of Jatropha. CRY2,CUC2, PIN1, TAA1 and CKX1 genes were identified for female flowering and SUP gene for transition of female flower. Further, RNA-seq based comprehensive transcriptome sequencing of inflorescence meristems (treated with cytokinin) and control (untreated inflorescence meristems) at time-intervals of 15 and 30 days, was performed. Through KEGG annotation, metabolic pathways were mapped to photosynthesis, carbon fixation, carbohydrate metabolism and nitrogen metabolism. These pathways were upregulated post cytokinin treatment (15 days) however, after 30 days, genes involved in these pathways were observed to be downregulated. We observed significant downregulation of genes FBP, SBP, GS, GDHandAGPase genes weredownregulated post 30 days of cytokinin treatment.
Pagination: xxv, 120p.
URI: http://hdl.handle.net/10603/218730
Appears in Departments:Department of Biotechnology

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02_certificate, declaration, acknowledgement.pdf1.04 MBAdobe PDFView/Open
03_table of contents;list of tables & figures, abbr, abstract.pdf2.14 MBAdobe PDFView/Open
04_chapter 1.pdf672.81 kBAdobe PDFView/Open
05_chapter 2.pdf1.3 MBAdobe PDFView/Open
06_chapter 3.pdf1.42 MBAdobe PDFView/Open
07_chapter 4.pdf2.85 MBAdobe PDFView/Open
08_chapter 5.pdf670.37 kBAdobe PDFView/Open
09_summary.pdf449.04 kBAdobe PDFView/Open
10_future prospects.pdf482.07 kBAdobe PDFView/Open
11_references.pdf610.18 kBAdobe PDFView/Open
12_appendices.pdf462.96 kBAdobe PDFView/Open
13_list_of_publications.pdf500.99 kBAdobe PDFView/Open
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