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http://hdl.handle.net/10603/209104
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DC Field | Value | Language |
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dc.coverage.spatial | ||
dc.date.accessioned | 2018-07-23T04:42:46Z | - |
dc.date.available | 2018-07-23T04:42:46Z | - |
dc.identifier.uri | http://hdl.handle.net/10603/209104 | - |
dc.description.abstract | Allicin, an extremely active constituent of freshly crushed garlic, is produced upon reaction of substrate alliin with the enzyme alliinase (EC 4.4.1.4). Allicin has been shown to be toxic to several mammalian cells in vitro in a dose-dependent manner. In the present study this cytotoxicity was taken to advantage to develop a novel approach to cancer treatment, based on site directed generation of allicin. A bacterium Cupriavidus necator with the ability of alliinase production was isolated newlinefrom soil sample and was identified by morphological, biochemical and 16S rRNA newlinesequence. Alliinase production was optimised and it was further purified to apparent homogeneity with 103-fold purification and specific activity of 209 U/mg of protein by using DEAE Cellulose and Sephadex G-100 chromatography. The enzyme is a homodimer of molecular weight 110 kDa with two subunits of molecular weight 55 kDa each. The optimum activity of the purified enzyme was found at pH 7 and the optimum temperature was 35 oC. The enzyme exhibited maximum reaction rate (Vmax) at 74.65 U/mg and Michaelis Menten constant (Km) was determined to be 0.83 mM newlinewhen alliin was used as a substrate. newline | |
dc.format.extent | xxv, 237 | |
dc.language | English | |
dc.relation | ||
dc.rights | university | |
dc.title | IN SITU ALLICIN GENERATION USING TARGETED ALLIINASE DELIVERY FOR INHIBITION OF MIA PaCa 2 CELLS AND DETERMINING RELEVANT MECHANISMS RESPONSIBLE FOR INHIBITION | |
dc.title.alternative | ||
dc.creator.researcher | CHHABRIA VIJAY SAGAR | |
dc.subject.keyword | Diagnosis | |
dc.subject.keyword | Human Pancreas | |
dc.subject.keyword | Molecular Genetics of PDAC | |
dc.subject.keyword | Pancreatic Cancer | |
dc.subject.keyword | Pathophysiology | |
dc.subject.keyword | Pungent Panacea Garlic | |
dc.subject.keyword | Staging/Classification of Tumour | |
dc.description.note | ||
dc.contributor.guide | DESAI B. KRUTIKA | |
dc.publisher.place | Mumbai | |
dc.publisher.university | Narsee Monjee Institute of Management Studies | |
dc.publisher.institution | Department of Biological Sciences | |
dc.date.registered | 30/04/2011 | |
dc.date.completed | 2016 | |
dc.date.awarded | ||
dc.format.dimensions | ||
dc.format.accompanyingmaterial | DVD | |
dc.source.university | University | |
dc.type.degree | Ph.D. | |
Appears in Departments: | Department of Biological Sciences |
Files in This Item:
File | Description | Size | Format | |
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01_ title page.pdf | Attached File | 164.46 kB | Adobe PDF | View/Open |
02_ declaration by the student.pdf | 255.78 kB | Adobe PDF | View/Open | |
03_ certificate.pdf | 252.54 kB | Adobe PDF | View/Open | |
04_ acknowledgements.pdf | 246.39 kB | Adobe PDF | View/Open | |
05_ abstract.pdf | 197.98 kB | Adobe PDF | View/Open | |
06_ contents.pdf | 1.58 MB | Adobe PDF | View/Open | |
07_ chapter 1 .pdf | 5.51 MB | Adobe PDF | View/Open | |
08_ chapter 2 .pdf | 804.68 kB | Adobe PDF | View/Open | |
09_ chapter 3 .pdf | 6.8 MB | Adobe PDF | View/Open | |
10_ chapter 4.pdf | 3.46 MB | Adobe PDF | View/Open | |
11_ chapter 5.pdf | 6.45 MB | Adobe PDF | View/Open | |
12_ chapter 6.pdf | 2.08 MB | Adobe PDF | View/Open | |
13_ chapter 7.pdf | 18.54 MB | Adobe PDF | View/Open | |
14_ chapter 8 .pdf | 393.79 kB | Adobe PDF | View/Open | |
15_ references.pdf | 10.05 MB | Adobe PDF | View/Open |
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