Evaluation of safety and preclinical efficacy of MD1 a poly herbal health supplement used in the management of diabetes mellitus

Abstract

MD1 a dietary supplement indicated in the management of Diabetes Mellitus is constituted of dried powdered herbs of repute as anti-diabetics in Indian traditional medicine MD1 was evaluated for residual toxins as per Ayurvedic Pharmacoepia of India procedures The effect of MD1 extract was studied for anti oxidant activity carbohydrate digestive enzyme inhibition AGE inhibitory potential MD1 effect on glucose and lipid metabolism was studied in L6 myotubes and 3T3L1 preadipocytes The in vivo effects of MD1 and its plausible mechanisms of action were studied in High Fat Diet induced Diabetes Mellitus in C57BL6J mice Residual toxins present within the API limits and MD1 demonstrated strong antioxidant potential and significantly inhibited the gama glucosidase and gama amylase activity MD1 significantly p less than 0 point 05 enhanced the insulin stimulated glucose uptake in both the cell lines studied. Unlike standard pioglitazone MD1 modulated the mRNA expression of PPARand#947; and GLUT4 p lessthan 0 point 0001 in 3T3L1 adipocytes, without inducing adipogenesis. Three weeks of MD1 administration significantly reduced the elevated fasting blood glucose triacylglycerol and total cholesterol in HFD fed mice MD1 protected against HFD induced weight gain while caloric intake remained unchanged MD1 treatment improved adipose tissue functioning by reducing TNF gama infiltration followed by enhanced mRNA expression of PPARy and Glut4 Histological and biochemical observations showed attenuation of HFD induced fatty liver disease due to MD1 through reduction of oxidative stress and TAG accumulation demonstrating a possible two hit mechanism The findings on glycemic intervention of MD1 supporting its use as a health supplement in the management of diabetes mellitus newline