Biological evaluation of dipeptide coated magnetic nanoparticles in lung cancer models under pulsed electromagnetic field

Abstract

newlineThe purpose of the study was to establish enhanced cellular uptake of dipeptide coated magnetic nanoparticles in vitro and in vivo in lung tumor cells and to test the suitability of the magnetic nanoparticles in inducing localized hyperthermia in a pulsed electromagnetic field magnetic nanoparticles were synthesized by co precipitation method and characterized by transmission electron microscopy dynamic light scattering and vibration sample magnetometer the effect of the magnetic nanoparticles on cellular viability was assessed in and was found to be non cytotoxic to lung cancer cells In vitro cellular uptake of the magnetic nano particles in lung cancer cells and in vivo bio distribution in a tumor xenograft model were confirmed by Prussian blue staining and inductively coupled plasma optical emission spectrophotometer the magnetic nanoparticles were tested for heating effects in a pulsed electromagnetic field and its effect on cellular morphology viability and expression of heat shock and apoptotic markers were assessed in lung cancer cells the magnetic nanoparticles in the presence of induced cell death in lung cancer cells