Isolation Pharmacological Evaluation and Formulation Develppment of Bio Active Princple from Crude Oryza Sative Bran oil

Abstract

In recent years, naturally occurring phytochemicals with antioxidant capacity have generated newlinesurmount interest for their therapeutic usage against a wide range of pathophysiological newlineconditions, most of which involve oxidative deterioration induced by free radicals. Therefore, the newlinepresent study was designed to isolate oryzanol (OZ), a bio-active natural antioxidant from crude newlineOryza sativa bran oil (cRBO) using an optimized technique, followed by its pharmacological newlineinvestigation, strategic formulation development and subsequent in vivo pharmacokinetic newlineevaluation. newlineThe physicochemical properties, fatty acid profile, and tocopherol/tocotrienol composition of the newlinecommercially obtained cRBO were comparable to those reported in the literature. The isolated newlineOZ from cRBO by a two-step crystallization process was identified by several analytical newlinetechniques. newlineIn the present study, oral administration of OZ (50 and 100 mg/kg, p.o.) reduced the blood newlineglucose level in normal and in streptozotocin (STZ)-induced diabetic rats in both single and newlinemultidose study (14 days). Oxidative stress produced by STZ was found to be significantly newlineameliorated by OZ when compared to the control rats. In addition, chronic treatment with OZ for newline8 weeks significantly improved the renal function and attenuated the behavioral as well as newlinebiochemical changes associated with diabetic nephropathy and neuropathy respectively in a newlinedose-dependent manner. Histopathological observations also evidenced regression in renal newlinepathological alterations with OZ and were comparable to glibenclamide (Gl) (10 mg/kg, p.o.). newlineFurther, OZ strongly inhibited the proliferation of colon and liver cancer cells in-vitro by a newlinemechanism that involved concentration dependent cytotoxicity. The predominant form of cell newlinedeath was likely by the induction of apoptosis. In-vivo findings suggested that chronic newlineadministration of OZ (100 mg/kg, p.o.) throughout an entire period of 32 weeks to carcinogenexposed newlineBalb/c mice ameliorated the deleterious effects of 1, 2 dimethyl hydra