Evaluation of Phytochemical and Anti Diabetic Potential of Three Sources of Mayurasikha An Ayurvedic Drug

Abstract

newlinePlants have formed the basis for traditional medicine system in various cultures since newlineancient time and plant based medicinal system plays a vital role in the present newlinesynthetic era. The synonyms of herbal drugs have caused controversy in their newlineidentification. Mayurasikha which is used for diabetes has three ambiguous sources newline(Adiantum caudatum, Celosia argentea and Actiniopteris dichotoma) in literature. newlineHence, there is a need to establish precise source of the plant to achieve the newlinetherapeutic uses by detailed study of quality control parameters, phytochemical newlineprofiling and pharmacological investigation. newlineObjective: The present study is carried out to establish the quality control parameters newlineas per WHO guidelines and comparative evaluation of phytoconstituents and anti- newlinediabetic potency among three plants. newlineMethodology: Various morphological, microscopical, physicochemical parameters newlineand comparative TLC, HPTLC, and HPLC of all the three plant extracts were studied newlineaccording to the WHO and pharmacopoeial guidelines. Alcoholic extracts of three newlineplants were fractionated and subjected to both in vitro (and#945;-amylase and and#945;-glucosidase newlineinhibitory activity and glucose uptake activity in L-6 cell lines.) and in vivo (high fat newlinediet + low dose streptozotocin induced type 2 diabetes) antidiabetic studies based on newlinebio activity guided isolation. newlineResults: All the three plants were clearly distinguished based on their morpho- newlineanatomical features along with different physico-chemical properties. A.dichotoma newlineshowed high amount of water soluble ash. A. dichotoma and A. caudatum showed the newlineKLE University, Belagavi. xvii newline newline4. Results newline newlineabsence of Saponins. C. argentea was rich in total poly phenols and flavonoids. The newlineTLC and HPTLC analysis indicated presence of different chemical constituents with newlinevarious R newlinef newline values and certain common chemical constituents with same R newlineHPLC method provided valid information about similarities in their chemical newlinecharacterization and differences in contents of marker c