Pharmacological evaluation of Cassia tora Brassica campestris and Calotropis procera on cognitive function in normal and diabetic rats

Abstract

newline The present study aimed to evaluate cognition enhancing effect of Cassia tora newlineleaves, Brassica campestris seed extract and Calotropis procera latex in normal rats newlineusing scopolamine and ECS induced amnesia model and in STZ induced diabetic rats. newlineMale wistar rats were pre-treated with high, medium and low dose of C. tora newlineleaves, B. campestris seed extract and C. procera dry latex in scopolamine induced newlineamnesia model. The dose having maximum effect on cognitive performance was selected newlineand further evaluated using scopolamine and electroconvulsive shock induced amnesia newlinemodel and also in STZ induced diabetic rats using behavioral and biochemical parameters. newlineThe behavioral performance was evaluated by elevated plus maze, step through passive newlineavoidance and Morris water maze, while biochemical estimation of acetylcholinesterase newlineactivity, and#946; amyloid newline1-42 newlineconcentration, dopamine and nor adrenaline concentration from rat newlinebrain homogenate was done. Simultaneously, C. tora leaves, B. campestris seed extract newlineand C. procera dry latex was screened for in vitro acetylcholinesterase inhibition activity. newlineAlso, in silico molecular docking study was performed to find active phytoconstiuents newlineand its molecular interaction to inhibit acetylcholinesterase activity. newlineScreening of C. tora leaves, B. campestris seed extract and C. procera dry latex newlineusing high, medium and low dose, showed dose dependent increase in cognitive newlineperformance in scopolamine-induced amnesia. Further, selected dose of each plant newlineextract showed significant improvement in behavioral performance and in biochemical newlineestimation in both scopolamine and ECS induced model and in diabetic rats. In in vitro newlinemicroplate assay for AChE inhibition activity C. tora leaves, B. campestris seed extract newlineXVIII newlineand C. procera dry latex were found active at all concentration and showed dose newlinedependant inhibition of AChE activity. While in silico molecular docking study revealed newlinethat compounds from different classes like flavonoids, vitamin, cardenolides