Chromatographic Fingerprint Analysis of Vernonia Cinerea l And Elephantopus scaber l xtracts by HPTLC Technique and Evaluation for Their Antiurolithiatic Potentials in Rats

Abstract

newlineBackground: Interlinking of secondary metabolite profiling and botanical identification of plant would be an efficient and potential tool in estimation of genetic variations as well as determination of phytochemical markers in numerous plant species. In past few years, quality assessment and control of complex herbal products was achieved by developing fingerprints with help of advanced chromatographic techniques. Urolithiasis is reported at a ratio of 1:20 persons at one or the other time throughout their life span. newlineAim and Objectives: The study undertaken was to develop the fingerprints of hydro-alcoholic extracts of Vernonia cinerea L. and Elephantopus scaber L. Further, an attempt to provide a scientific base to validate the claims made and use of Vernonia cinerea L. and Elephantopus scaber L. in management and treatment of kidney stones followed by evaluation of anti-oxidant and antimicrobial potential. newlineMethodology: The plants namely Vernonia cinerea Less and Elephantopus scaber Linn were collected from surrounding areas of Belagavi and authenticated. TFor extraction cold maceration [95% ethanol:water (70:30)] method was used with occasional shaking for seven days. These extracts were used to develop finger prints by HPTLC technique. Ethylene glycol was used to induce kidney stone in rats for screening of antiurolithiatic activity. Also, antimicrobial activity was studied against four uropathogens. DPPH was used to evaluate antioxidant potentials. newlineResults: V. cinerea showed 11 alkaloids, 32 flavonoids, 26 saponins, 20 sterols, 23 steroids, 20 glycosides, 22 tannins, 20 triterpenoids and 31 phenols present; whereas E. scaber revealed presence of 12 alkaloids, 30 flavonoids, 27 saponins, 20 sterols, 21 steroids, 23 glycosides, 17 tannins, 17 triterpenoids and 30 phenols with different Rf values after scanning at a wavelength of 254, 366 and 540 nm inclusive of known and unknown secondary metabolites. newlineAnimals treated with extracts showed promising results when screened for their anti-urolithiati