Please use this identifier to cite or link to this item:
http://hdl.handle.net/10603/19542
Title: | Cloning sequence analysis and characterization of DNA polymerase from Geobacillus sp. WBI |
Researcher: | Nayak, Shreenath |
Guide(s): | Biswas, Swadesh Ranjan |
Keywords: | Geobacillus Sequence analysis Thermostable DNA polymerase |
Upload Date: | 23-Jun-2014 |
University: | Visva Bharti University |
Completed Date: | n.d. |
Abstract: | A thermostable DNA polymerase gene (DNA pol) was isolated from the genomic DNA of Geobacillus sp.WBI by polymerase chain reaction (PCR). PCR amplification with the primer pair designed from the conserved sequence of several Geobacillus spp. produced a single band of 1.9 kb DNA fragment. The PCR product was purified and sequenced. Nucleotide sequence analysis revealed a high degree of similarity with the corresponding sequences of thermophilic Bacillus adolyticus XM, Geobacillus thermoleovorans, Bacillus caldotenax, Geobacillus kaustophilus, Bacillus sp. G and Geobacillus sp. Y412MC52. The maximum similarity (99%) was shared by B. caldolyticus. Based on the sequence comparison, it was found that the organism contained a thermostable DNA pol in its genome. Since the thermostable DNA polymerase is extremely important biotechnologically, the present study encourages further research to fully characterize the enzyme and determine its potential applications. |
Pagination: | iii, 171p. |
URI: | http://hdl.handle.net/10603/19542 |
Appears in Departments: | Department of Botany |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
01. title.pdf | Attached File | 25.87 kB | Adobe PDF | View/Open |
02. dedication.pdf | 19.21 kB | Adobe PDF | View/Open | |
03. certificate.pdf | 273 kB | Adobe PDF | View/Open | |
04. acknowledgement.pdf | 12.22 kB | Adobe PDF | View/Open | |
05. content.pdf | 2.46 kB | Adobe PDF | View/Open | |
06. introduction.pdf | 51.1 kB | Adobe PDF | View/Open | |
07. review of literature.pdf | 573.62 kB | Adobe PDF | View/Open | |
08. chapter 1.pdf | 5.29 MB | Adobe PDF | View/Open | |
09. chapter 2.pdf | 1.83 MB | Adobe PDF | View/Open | |
10. chapter 3.pdf | 1.69 MB | Adobe PDF | View/Open | |
11. discussion.pdf | 29.5 kB | Adobe PDF | View/Open | |
12. refrence.pdf | 102.52 kB | Adobe PDF | View/Open | |
13. publication.pdf | 14.05 kB | Adobe PDF | View/Open |
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