Exploring the antidiabetic potential of Myxopyrum serratulum A W Hill and Nilgirianthus ciliatus Nees derived nanoparticles

Abstract

Diabetes mellitus is the most common endocrine disorder caused either by insufficient insulin secretion or insulin resistance Existing oral anti-diabetic medications address hypoglycemia but leads to many side effects In addition to lack of efficacy and undesirable adverse effects of synthetic drugs herbal drugs are prescribed throughout the world However the major drawback is that the quantity of herbal extract necessary for treatment is higher due to the degradation of different plant constituents in the acidic pH of the stomach which promotes the loss of the desired effect and a longer duration of treatment is needed due to the poor absorption of these constituents in the intestine Recently, many studies focused on applying nanotechnology to plant extracts for the enhancement of solubility bioavailability and sustained delivery Hence the present study was designed to incorporate nanotechnology to medicinal plants MS and NC and investigate the antidiabetic effect of Medicinal plants and prepared nanoparticles GMSN and GNCN by in vitro and in vivo methods While MS and GMSN2 exhibited better activity in all in vitro studies compared to NC and GNCN3 Hence MS and GMSN2 were selected to confirm the antidiabetic effect by HFD induced diabetes in C57BL 6J mice The study revealed that GMSN2 at low dose 80 mg and 160 mg kg bwt significantly decrease the blood glucose triglycerides cholesterol increase the antioxidant enzymes and reverse the carbohydrate metabolic enzyme levels in liver of diabetes induced C57BL 6J mice This can be attributed due to the characteristics effect of sustained release of active constituents in the nanoparticles This release is due to the inclusion of gelatin polymer in the preparation of nanoparticles Thus we suggest that the nanoparticles system can be applied to overcome other water poorly soluble herbal medicines and furthermore to decrease the treatment dosage This study could serve as a useful reference to allow the future exploitation of nanoparticulate system as a novel preventive and