Synthesis Characterization And Microbial Evaluation of Novel Pyrazoles and Oxadiazoles Derivatives

Abstract

NMR Spectra were recorded on 500 MHz, 400 MHz and 300 MHz Varian instrument newlineand Avance 300 MHz Bruker UX NMR instrument with TMS as internal standard newlineand the chemical shifts are expressed in and#61540; values. The abbreviations such as s, d, t, m, newlinedd, bs, refers to singlet, doublet, triplet, multiplet and doublet of doublet, broad singlet newlinerespectively. newline2. Mass spectra were recorded on VG Micromass 7070 H (EI and CI), VG Autospec newline(FAB) using Cs+ ion gun, m-nitro benzyl alcohol (MNBA) as a matrix and Finnigan newlineMat 1020 Mass (GC-MS) instruments. newline3. IR spectra were recorded on Perkin Elmer Model 283B and Nicolet-740 FT-IR newlineinstruments and values are given in cm-1. newline4. Melting points were recorded on Superfit make melting point apparatus and newlineare uncorrected. newline5. All solvents and reagents were purified by standard techniques. Evaporation of newlinesolvents was carried out under reduced pressure on Hedolph rotary evaporator below newline45 °C. newline6. Analytical thin layer chromatography (TLC) was performed on precoated silica gel-60 newlineF254 (0.5 mm) glass plates. Visualization of the spots on TLC plates was achieved newlineeither by exposure to iodine vapor or UV light or by dipping the plates to sulphuric newlineacid-and#61537;-napthol or to ethanolicanisaldehyde-sulphuric acid-acetic acid or to newlinephosphomolybdic acid-sulphuric acid solution and heating the plates at 1200C. newlineColumn chromatography was performed using silica gel (60-120 and 100-200 mesh) newlineand the column was usually eluted with ethyl acetate-petroleum ether newline