Invivo evaluation of protein supplement in the management of Uremia

Abstract

Chronic Kidney Disease is the worldwide public health problem. It is the 12th cause of disability and 18th newlinecause of mortality among other disease. Uremia is the condition which leads to severe metabolic consequences results newlinein severe mortality in kidney disease subjects. Dietary protein plays a vital role in the management if kidney disease. newlineObjective: The aim of this study is to formulate the high protein cookies incorporated with egg white powder and to newlinedetermine the ileal digestibility of Indispensible amino acid for the formulated high protein cookies in uremic female newlinewistar rats. Methods: ninety two wistar rats of age 8-12 weeks were selected for the study. The rats were grouped as newlinecontrol and uremic group. Neprhrotoxicity was induced in wistar rats by administration of gentamicin 80 mg/g/day newlineintraperitoneally, The rats were fed with cookies for 14 day period. Each day each rat received its diet for 10 minutes newlineperiod as nine meals given at hourly intervals (0800-1600 hour). On the 14th day, from 5-7 hours, the rats were newlineasphyxiated with carbon di oxide and decapitated. The 20 cm of ileum immediately anterior to ileo-ceacal junction newlinewas dissected out and ileal contents were flushed using distilled water, freeze dried and subjected to chemical analysis. newlineResults: The formulated cookie and ilealdigesta were estimated for indispensible amino acid using HPLC. It was found newlinethat the rate of absorption of phenylalanine and methionine were found to be higher in uremic rats compared to the newlinenormal group compared to other IAA. Conclusion: From the study it is concluded that the protein absorption is better newlinein uremic rats and hence it can be recommended for subject with kidney disease. Hence kidney disease subjects should newlineconsume adequate high biological value protein to compensate the protein loss