Development of a topical herbal formulation for wound healing from selected fraction of Dodonaea viscosa L Jacq

Abstract

Dodonaea viscosa L Sapindaceae is an important folklore medicine used for malaria ulcers dysmenorrhoea rheumatism sprains bruises burns and wounds literatures showed the presence of flavonoids diterpenoid acids saponins P coumarin acid ester sterols essential oils and tannins Based on the folklore claim and the phytoconstituents present the plant Dodonaea viscosa was selected for the study to explore the wound healing potential of the plant In the present study the pharmacognostical physicochemical and elemental analysis was carried out to authenticate the plant material Various fractions were prepared from leaves and phytochemical constituents were analyzed and quantified Invitro antioxidant and antimicrobial studies were carried out to select the effective fraction which was further evaluated for invitro wound healing activity by fibroblast proliferation assay and cell migration assay Topical formulation has been developed with ethyl acetate fraction DVFO and evaluated for invivo wound healing activity in animal model The results revealed that the developed formulation DVFO showed significant wound healing potential in a dose dependent manner compared with control group The wound healing potential of the formulation DVFO may be attributed to the presence of phenols flavonoids and tannins present in the ethyl acetate fraction of Dodonaea viscosa newline